CHENG Rui, LIANG Chang, NAN Fang, HU Zhi, VLAK M and CHEN Xin-wen. Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus[J]. Virologica Sinica, 2003, 18(6): 587-592.
Citation:
CHENG Rui, LIANG Chang, NAN Fang, HU Zhi, VLAK M, CHEN Xin-wen.
Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus .VIROLOGICA SINICA, 2003, 18(6)
: 587-592.
SeMNPV抑制HaSNPV诱导的Se—UCR细胞凋亡
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1.
中国科学院武汉病毒研究所，分子病毒学重点实验室，湖北武汉，430071，china
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2.
Lab of Virology，Wageningen University,Binnmhavm 11。6709PD,the Nedmtmds
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摘要
摘要：在采用共感染和共转染的方法构建扩大杀虫范围的重组病毒的研究过程中发现棉铃虫单核衣壳核多角体 病毒(Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus。HaSNPV)能诱导甜菜夜蛾细胞Se-UCR发生典 型凋亡，但不能诱导另一株甜菜夜蛾细胞Se．301产生凋亡。以5 MOI的HaSNPV感染Se-UCR。在12h左右可 以观测到少量细胞凋亡。24h能观察到明显的凋亡，凋亡细胞数量随时间不断增加，到72h基本上所有的细胞均 发生凋亡，成为凋亡小体，基因组DNA片段化。同时发现HaSNPV诱导的甜菜夜蛾Se-UCR细胞凋亡能够被甜 菜夜蛾多核衣壳核多角体病毒(Spodoptera exigua multicapsid nucleoplyhedrovirus，SeMNPV)所抑制， 进一步点杂 交试验发现SeMNPV 和HaSNPV共同感染Se．UCR获得了HaSNPV在该细胞中的复制。
Abstract：Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodopteraexigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides．Aspe sticides，both viruses have very narrow host range and Can not make cross infection．Trying tOgenerate a rec ombinant baculovirus with broader host range by CO—infection and co-transfection，wefound that HaSNPV could trigger apoptosis of a exigua cell line Se·UCR．which could be blocked bySeM NPV，but apoptosis did not occur in Se一30 1，a cell line also from exigua．W hen infected Se-UCRwith 5M OI，theapoptoticbodywasIn'stfound at 12hpi，obvious apoptosis couldbe observedat 24hpi，at the end almost all ceHs were dead at 72 hpi．Those observations were confu-med by genome DNAfragmen-ration analysis．Dot blot an alysis indicated that SeMNPV could also help HaSNPV genomeDNA an d then virus repficafion in Se-UCR cells，but not in Se-301．
Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus
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1.
1．KeyLab MokcuI口r vimlogy．WuhanInstituteofvimlogy．ChineseAcademyofSciences,Wuhan430071．China
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2.
Lab of vimlogy．Wageningen University．Binnenhaven 11。6709PD Wageningen,the Netherlands
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Abstract
Abstract：Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides．As pe sticides，both viruses have very narrow host range and Can not make cross infection．Trying tO generate a rec ombinant baculovirus with broader host range by CO—infection and co-transfection，we found that HaSNPV could trigger apoptosis of a exigua cell line Se·UCR．which could be blocked by SeM NPV，but apoptosis did not occur in Se一30 1，a cell line also from exigua．W hen infected Se-UCR with 5M OI，theapoptoticbodywasIn’stfound at 12hpi，obvious apoptosis couldbe observedat 24hpi， at the end almost all ceHs were dead at 72 hpi．Those observations were confu-med by genome DNA fragmen-ration analysis．Dot blot an alysis indicated that SeMNPV could also help HaSNPV genome DNA an d then virus repficafion in Se-UCR cells，but not in Se-301．
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