摘要：通过PCR扩增，获得OaEPV球状体蛋白基因编码区序列，并进行克隆、测序。分析结果显示，OaEPV球 状体蛋白基因编码区全长为2967bp，编码分子量为l1lkDa的球状体蛋白。同源性分析表明，OaEPV球状体蛋白 基因与直翅目昆虫痘病毒关系最近，与鳞翅目和鞘翅目昆虫痘病毒关系较远。对已知的几种昆虫痘病毒球状体蛋 白基因做系统进化树，结果显示，以病毒寄主的昆虫分类目作为昆虫痘病毒的分属依据，更符合分子水平的分析 结果，也与近年来许多学者所提出的“将鳞翅目与直翅目昆虫痘病毒分为2个不同的属”的观点相一致。对这些 球状体蛋白氨基酸序列的疏水性进行分析，表明球状体蛋白的疏水性随寄主昆虫所处的目不同而表现出较大差 异，这可能是因为病毒与寄主长期的协同进化的结果。 Abstract：TIle coding region of Oedaleus asiaticus EPV spheroidin gene was obtained by PCR．and thisfragment Was subsequently cloned，seq uenced an d an alyzed．Tlle data shows OaEPV sph coding re onis 2967bp in size which encodes a ll l l(Da protein．Homology an alysis demonstrated that 0aEPV iscloser to Orthoptcran EPVS than Lepidopteran an d Coleopteran EPVS．Phylogenetic tree derived fromall known EPVS spheroidin gene indicates that the order of the EPV host is in relationship with thegenus of corresponding EPV．which agres wit|l a recent point that “Lepidopteran EPVs an dOrthopteran EPVS should be divided into two distinct genus”．Hydrophobicity plots of these kn ownsperoidin show that the more distan t relationship of the orders the viruses hOsts be long to．the morediverse of the property of EPV spheroidin to each other．It may be explained as the result ofCO．evolutionbe tweentheEPVSan dtheirhos

Abstract：TIle coding region of Oedaleus asiaticus EPV spheroidin gene was obtained by PCR．and this fragment Was subsequently cloned，seq uenced an d an alyzed．Tlle data shows OaEPV sph coding re on is 2967bp in size which encodes a ll l l(Da protein．Homology an alysis demonstrated that 0aEPV is closer to Orthoptcran EPVS than Lepidopteran an d Coleopteran EPVS．Phylogenetic tree derived from all known EPVS spheroidin gene indicates that the order of the EPV host is in relationship with the genus of corresponding EPV．which agres wit|l a recent point that “Lepidopteran EPVs an d Orthopteran EPVS should be divided into two distinct genus”．Hydrophobicity plots of these kn own speroidin show that the more distan t relationship of the orders the viruses hOsts be long to．the more diverse of the property of EPV spheroidin to each other．It may be explained as the result of CO．evolutionbe tweentheEPVSan dtheirhos