Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies

XU Li; WANG Ling; SU Xin; WAN G; W U; CHEN Pu

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December 2003

XU Li, WANG Ling, SU Xin, WAN G, W U and CHEN Pu. Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies[J]. Virologica Sinica, 2003, 18(6): 614-615.

Citation: XU Li, WANG Ling, SU Xin, WAN G, W U, CHEN Pu. Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies .VIROLOGICA SINICA, 2003, 18(6) : 614-615.

猪繁殖与呼吸综合征与伪狂犬病混合感染的分子生物学快速诊断

1.
1．南京农业大学农业部畜禽疫病诊断与免疫重点开放实验室，江苏南京，210095
2.
宁夏大学农学院动物科学系，宁夏银川，750105
3.
农业部动物检疫所，山东青岛，266032

摘要

猪繁殖与呼吸综合征自1987年发现至今，已在世界大多数养猪国家和地区普遍存在【1．2】。而伪狂犬病作为仅次于口蹄疫、猪瘟的危害养猪业的重要疫病，一直引起人们的高度重视【3．4l。这两种传染病在猪群中发生以后，同样出现妊娠母猪流产、产木乃伊胎等繁殖障碍，从流行病学、临床症状等方面也难以做出判断。 Abstract：According to the nucleotide sequences of Porcine Reproductive and Respiratory Synd romeV／ms VR2332 strain and Pseudorabies virus shan ghai strain provided in GenBank， two pairs ofprimersweredesignedto amplifytheN geneofPRRSV byRT—PCR an dTkgeneofPRV byPCR． Asa result， a rapid molecular diagn osis with high specificity an d accuracy was set up．The resultindicated that PRRSV Was detected from 11 out of 33 samples， PRV was detected from 9 out of 33sam ples， an d CO—infec tion by PRRSV an d PRV was confirmed in 8 samples， the rate of co-infectionWas at24．2％．
- 猪繁殖与呼吸综合征
- , 伪狂犬病
- , 混合感染
- , 快速诊断

Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies

1.
1．KeyLaboratoryofAnimalDiseaseDiagnosisandImmunologyNanjingAgricultural University,Nanjing 210095． China
2.
Department ofAnimal Science．Agricultural College ofNingxia University．Yinchuan 750105．China 3 Animal Quarantine Institute of Agriculture Ministry,Qingdao 266032，China

Abstract

Abstract：According to the nucleotide sequences of Porcine Reproductive and Respiratory Synd rome V／ms VR2332 strain and Pseudorabies virus shan ghai strain provided in GenBank， two pairs of primersweredesignedto amplifytheN geneofPRRSV byRT—PCR an dTkgeneofPRV byPCR． As a result， a rapid molecular diagn osis with high specificity an d accuracy was set up．The result indicated that PRRSV Was detected from 11 out of 33 samples， PRV was detected from 9 out of 33 samples， and CO—infec tion by PRRSV an d PRV was confirmed in 8 samples， the rate of co-infection Was at24．2％．
- Porcine Reproductive an d Respiratory Syndrome
- , Pseudorabies
- , Concurrent infection
- , Rapid diagnosis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies

1. 1．KeyLaboratoryofAnimalDiseaseDiagnosisandImmunologyNanjingAgricultural University,Nanjing 210095． China
2. Department ofAnimal Science．Agricultural College ofNingxia University．Yinchuan 750105．China 3 Animal Quarantine Institute of Agriculture Ministry,Qingdao 266032，China

Abstract: Abstract：According to the nucleotide sequences of Porcine Reproductive and Respiratory Synd rome V／ms VR2332 strain and Pseudorabies virus shan ghai strain provided in GenBank， two pairs of primersweredesignedto amplifytheN geneofPRRSV byRT—PCR an dTkgeneofPRV byPCR． As a result， a rapid molecular diagn osis with high specificity an d accuracy was set up．The result indicated that PRRSV Was detected from 11 out of 33 samples， PRV was detected from 9 out of 33 samples， and CO—infec tion by PRRSV an d PRV was confirmed in 8 samples， the rate of co-infection Was at24．2％．