选用产EB病毒的绒猴淋巴细胞B95-8系和补体受体2型 (complement receptor 2, CR2)与多聚免疫球蛋白受体(polymeric immunoglobulin receptor, pIgR)表达阴性的人永生化上皮细胞Hacat系共培养，进行细胞接触感染实验。一周后去除B95-8细胞，仅留Hacat细胞，并以自行改进的方法鉴定前者是否得以彻底去除。在证实没有B95-8残留后，PCR和原位杂交分别检验剩余Hacat细胞中EB病毒的感染结果。实验结果表明：改进的方法能够灵敏和简便地判断B95-8细胞的污染与否，并且与B95-8细胞接触共培养的Hacat细胞能被EB病毒有效地感染，后者暗示了EB病毒对上皮细胞可能存在细胞融合和CR2或pIgR介导之外新的感染途径。本研究在一定程度上简化了前人的细胞接触感染方法，也为建立天然的EB病毒自发有效地感染上皮细胞的模型奠定了基础。

Human immortal epithelial cell line-Hacat cells without expression of CR2(complement receptor 2, CR2) and polymeric immunoglobulin receptor(pIgR) were co-cultivated with marmoset lymphocyte line-B95-8 cells producing EBV for infection by cell-to-cell contacting. After a week, all the B95-8 cells were removed to keep only Hacat cells, and an ameliorated method was applied to determine whether the former was eradicated or not. When there were no contaminated B95-8 cells in those residual Hacat cells, EBV infection was detected and confirmed by Polymerase Chain Reaction (PCR) and in situ Hybridization(ISH), respectively. The results showed that the ameliorated method was sensitive, simple and convenient for detecting the contamination of B95-8 cells, and the Hacat cells can be effectively infected by EBV when co-cultivated with B95-8 cells, suggesting that there is a new pathway other than cell-to-cell fusing and CR2 or pIgR mediating for EBV to infect epithelial cells. This study simplified the method of infection via cell-to-cell contacting, and laid the foundation for establishing the model with EBV to spontaneously and effectively infect epithelial cells.