Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province

LI Lan-juan; LU Yi-yu; WENG Jing-qing; YAN Ju-ying; WU Nan-ping; WO Jian-er; SHI Wen; GONG Li-ming; ZHU Han-ping; GE Qiong; LI Min-hong; LU Qun-ying; CHENG Su-yun; ZHANG Yan-jun; YAO Ping-ping; MEI Ling-ling; WANG Zhi-gang; FEN Yan; MAO Hai-yan

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February 2004

LI Lan-juan, LU Yi-yu, WENG Jing-qing, YAN Ju-ying, WU Nan-ping, WO Jian-er, SHI Wen, GONG Li-ming, ZHU Han-ping, GE Qiong, LI Min-hong, LU Qun-ying, CHENG Su-yun, ZHANG Yan-jun, YAO Ping-ping, MEI Ling-ling, WANG Zhi-gang, FEN Yan and MAO Hai-yan. Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province[J]. Virologica Sinica, 2004, 19(1): 14-17.

Citation: LI Lan-juan, LU Yi-yu, WENG Jing-qing, YAN Ju-ying, WU Nan-ping, WO Jian-er, SHI Wen, GONG Li-ming, ZHU Han-ping, GE Qiong, LI Min-hong, LU Qun-ying, CHENG Su-yun, ZHANG Yan-jun, YAO Ping-ping, MEI Ling-ling, WANG Zhi-gang, FEN Yan, MAO Hai-yan. Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province .VIROLOGICA SINICA, 2004, 19(1) : 14-17.

浙江省SARS冠状病毒分离与系统进化树分析

李兰娟 ¹ ,
卢亦愚 ^2*,, ,
翁景清 ,
严菊英 ² ,
吴南屏 ¹ ,
沃健儿 ¹ ,
龚黎明 ² ,
朱函坪 ² ,
李敏红 ² ,
陆群英 ² ,
程苏云 ² ,
张严峻 ² ,
姚苹苹 ² ,
梅玲玲 ² ,
王志刚 ² ,
茅海燕 ² ,

1.
1. 浙江大学医学院，浙江杭州，310009
2.
浙江省疾病预防控制中心，浙江杭州 310009

通讯作者： 卢亦愚, ;

摘要

从浙江省3例SARS患者中收集含漱液标本，经处理后接种Vero、RD、VeroE6和Hep-2细胞进行病毒分离，培养3d后在Vero和RD细胞中可观察到细胞病变。从细胞培养上清中提取病毒核酸，用SARS冠状病毒特异性引物进行RT-PCR，并经测序证实从3份临床样本中分离到2株SARS冠状病毒株。对其中1株病毒的基因组进行了全序列测定并作系统进化树分析显示浙江省SARS冠状病毒株与新加坡2774株和台湾TW1株最为接近。
- SARS
- , 病毒分离
- , 序列
- , 系统进化树

Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province

1.
1. Medical College of Zhejiang University, Hangzhou 310009, China
2.
Zhejiang Center for Disease Prevention and Control

Corresponding author: LU Yi-yu,

Abstract

Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.
- SARS
- , Virus isolation
- , Sequence
- , Phylogenic tree

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province

Corresponding author: LU Yi-yu,

1. 1. Medical College of Zhejiang University, Hangzhou 310009, China
2. Zhejiang Center for Disease Prevention and Control

Abstract: Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.