WANG Xiao-chun, ZHANG Jia-min, LIU Chuan-feng, YV Hai-yang and HU Yuan-yang*. A New Picornavirus Isolated from Ectropis oblique[J]. Virologica Sinica, 2004, 19(1): 39-42.
Citation: WANG Xiao-chun, ZHANG Jia-min, LIU Chuan-feng, YV Hai-yang, HU Yuan-yang*. A New Picornavirus Isolated from Ectropis oblique .VIROLOGICA SINICA, 2004, 19(1) : 39-42.

从茶尺蠖中分离到一株微小RNA病毒

  • 从核型多角体病毒(NPV)感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒(EoPV)透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白,后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly(A)尾,编码RNA聚合酶(RdRp),含有微小RNA病毒RNA聚合酶的八个保守基序,同源性分析表明它与榕透翅毒蛾微小RNA病毒(PnPV)亲缘关系最近。这些特点表明该病毒为一株新的微小RNA病毒。

A New Picornavirus Isolated from Ectropis oblique

  • A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus. All of these properties indicate that this virus should be a member of Picornaviridae.

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    A New Picornavirus Isolated from Ectropis oblique

    • 1. Lab of Insect Virology, College of Life Science, Wuhan University, Wuhan 430072, China

    Abstract: A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus. All of these properties indicate that this virus should be a member of Picornaviridae.

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