Construction and Expression of HIV-1 Env Eukaryotic Expression Vector

WANG Fu-xiang; SUN Yong-tao*; WANG Lin-xu; WANG Jiu-ping; WANG Ping-zhong; BAI Xue-fan; HUANG Chang-xing

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February 2004

WANG Fu-xiang, SUN Yong-tao*, WANG Lin-xu, WANG Jiu-ping, WANG Ping-zhong, BAI Xue-fan and HUANG Chang-xing. Construction and Expression of HIV-1 Env Eukaryotic Expression Vector[J]. Virologica Sinica, 2004, 19(1): 70-72.

Citation: WANG Fu-xiang, SUN Yong-tao*, WANG Lin-xu, WANG Jiu-ping, WANG Ping-zhong, BAI Xue-fan, HUANG Chang-xing. Construction and Expression of HIV-1 Env Eukaryotic Expression Vector .VIROLOGICA SINICA, 2004, 19(1) : 70-72.

中国株HIV-1外膜蛋白真核表达载体的构建与表达

1.
第四军医大学唐都医院全军感染病诊疗中心，陕西西安 710038

摘要
- HIV-1
- , 外膜蛋白
- , 真核表达载体

Construction and Expression of HIV-1 Env Eukaryotic Expression Vector

1.
Center of Diagnosis and Treatment for Infectious Diseases of PLA. Tangdu Hospital, The Fourth Military Medical Univercity, Xi′an 710038, China

Abstract

To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence. Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine.
- HIV-1
- , Envelope protein
- , Eukaryotic expression vector

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction and Expression of HIV-1 Env Eukaryotic Expression Vector

1. Center of Diagnosis and Treatment for Infectious Diseases of PLA. Tangdu Hospital, The Fourth Military Medical Univercity, Xi′an 710038, China

Abstract: To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence. Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine.