Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif

ZHENG Lan-yan; LUO En-jie; LI Xin-ming; MOU Ling; ZHANG Xiao-tang; LU Run-ming

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February 2004

ZHENG Lan-yan, LUO En-jie, LI Xin-ming, MOU Ling, ZHANG Xiao-tang and LU Run-ming. Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif[J]. Virologica Sinica, 2004, 19(1): 73-75.

Citation: ZHENG Lan-yan, LUO En-jie, LI Xin-ming, MOU Ling, ZHANG Xiao-tang, LU Run-ming. Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif .VIROLOGICA SINICA, 2004, 19(1) : 73-75.

插入CpG序列的汉坦病毒S基因真核表达载体的构建及表达*

郑兰艳 ¹ ,
罗恩杰 ^1*,, ,
李新鸣 ¹ ,
张小棠 ² ,
鲁润铭 ² ,

1.
1. 中国医科大学基础医学院病原生物教研室, 辽宁沈阳 110001
2.
沈阳市传染病院中心研究室, 辽宁沈阳 110003

通讯作者： 罗恩杰, ;

摘要
- 汉滩病毒
- , 真核表达载体
- , 电穿孔
- , 瞬间表达

Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif

1.
1. Department of Microbiology & Parasitology, China Medical University, Shenyang 110001,China
2.
The Municipal Hospital of Infectious Disease, Shenyang 110003, China

Corresponding author: LUO En-jie,

Abstract

To improve the effect of the gene immunization against Hantaan virus, we constructed the eukaryotic expression vector pTARGET-hans(ISS) containing Hantaan Virus S gene coding region and CpG motif by cloning S gene segment with CpG motif into eukaryotic expression vector pTARGETTM. After conformed by enzyme analysis, the recombinant expression vector pTARGET-hans(ISS) was transferred into Vero-E6 cells by electroporation and the transient expression of Hantaan virus nucleocapsid protein was detected by indirect immunofluorescence assay(IFA). In some transferred Vero-E6, the green fluorescence was showed, thus we can conclude that the eukaryotic expression vector pTARGET-hans(ISS) was successfully constructed and expressed in vitro, which will lay a foundation for further animal vaccination.
- Hantaan virus
- , Eukaryotic expression vector
- , Electroporation
- , Transient expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif

Corresponding author: LUO En-jie,

1. 1. Department of Microbiology & Parasitology, China Medical University, Shenyang 110001,China
2. The Municipal Hospital of Infectious Disease, Shenyang 110003, China

Abstract: To improve the effect of the gene immunization against Hantaan virus, we constructed the eukaryotic expression vector pTARGET-hans(ISS) containing Hantaan Virus S gene coding region and CpG motif by cloning S gene segment with CpG motif into eukaryotic expression vector pTARGETTM. After conformed by enzyme analysis, the recombinant expression vector pTARGET-hans(ISS) was transferred into Vero-E6 cells by electroporation and the transient expression of Hantaan virus nucleocapsid protein was detected by indirect immunofluorescence assay(IFA). In some transferred Vero-E6, the green fluorescence was showed, thus we can conclude that the eukaryotic expression vector pTARGET-hans(ISS) was successfully constructed and expressed in vitro, which will lay a foundation for further animal vaccination.