Regulation of RU5 Region to Gene Expression of BFV 3026

KONG Xiao-hong; YU Hong; XUAN Cheng-hao; WANG Jin-zhong; CHEN Qi-min; GENG Yun-qi*

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April 2004

KONG Xiao-hong, YU Hong, XUAN Cheng-hao, WANG Jin-zhong, CHEN Qi-min, GENG Yun-qi* and Regulation of RU5 Region to Gene Expression of BFV 3026[J]. Virologica Sinica, 2004, 19(2): 129-132.

Citation: KONG Xiao-hong, YU Hong, XUAN Cheng-hao, WANG Jin-zhong, CHEN Qi-min, GENG Yun-qi*, . Regulation of RU5 Region to Gene Expression of BFV 3026 .VIROLOGICA SINICA, 2004, 19(2) : 129-132.

RU5区对BFV3026基因表达的调控*

1.
南开大学生命科学学院, 天津 300071

摘要

以牛泡沫病毒(Bovine foamy virus, BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。
- 牛泡沫病毒3026
- , RU5区
- , 基因表达调控

Regulation of RU5 Region to Gene Expression of BFV 3026

1.
College of Life Sciences, Nankai University, Tianjin 300071, China

Abstract

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.
- Bovine foamy virus
- , RU5 region
- , Gene expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Regulation of RU5 Region to Gene Expression of BFV 3026

1. College of Life Sciences, Nankai University, Tianjin 300071, China

Abstract: In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.