Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain

LIU Shu-ying; MA Xue-en; LI Jing-peng; ZHANG Jiu-he

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April 2004

LIU Shu-ying, MA Xue-en, LI Jing-peng and ZHANG Jiu-he. Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain[J]. Virologica Sinica, 2004, 19(2): 133-136.

Citation: LIU Shu-ying, MA Xue-en, LI Jing-peng, ZHANG Jiu-he. Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain .VIROLOGICA SINICA, 2004, 19(2) : 133-136.

绵羊肺腺瘤病毒中国NM株部分gag基因的克隆与序列分析*

刘淑英 ^1*,, ,
马学恩 ¹ ,
李景鹏 ² ,
张九河 ¹

1.
1. 内蒙古农业大学动物科学与医学学院, 内蒙古呼和浩特 010018
2.
东北农业大学生命科学学院基因部，黑龙江哈尔滨 150030

通讯作者： 刘淑英, ;

摘要

参照GenBank中已发表的绵羊肺腺瘤病毒的基因序列，设计合成一对引物，对绵羊肺腺瘤病毒（Jaagsiekte sheep retrovirus，JSRV）内蒙株的gag基因中主要编码CA蛋白的基因段进行PCR扩增，产物经琼脂糖凝胶电泳分析，呈现一条约897bp的特异条带，将其回收后克隆入pMD-18 T载体中，并进行序列测定与分析。结果表明，与南非株（基因序列号NC-001494）的gag基因序列比较，核苷酸同源性为83%，推导出的氨基酸同源性为84%。与美国株（基因序列号AF105220）的gag基因序列比较，核苷酸同源性为81.5%，氨基酸同源性为83%。这是我国首次报道的绵羊肺腺瘤病毒的gag基因的一段序列，为我国科研工作者进行更深入的研究奠定基础。
- 绵羊肺腺瘤病毒
- , gag基因
- , 克隆
- , 序列分析

Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain

1.
1. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China
2.
College of life science, Northeast Agricultural University, Harbin 150030, China

Corresponding author: LIU Shu-ying,

Abstract

In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, a pair of primers was designed according to the GenBank sequence . The fragment of gag gene was obtained by polymerase chain reaction (PCR), and the gene was cloned into PMD-18 T vector and identified by EcoR I and Sal I digestion. The nucleotide and amino acid sequences of NM strain partial gag gene were compared with the counterpart sequeuces of South Africa strain (Accession No. NC-001494) and USA strain (Accession No. AF105220). The nucleotide and amino acid homology of partial gag gene were 83%, 81.5% and 84%, 83%, respectively. This is the first JSRV nucleotide sequence reported in China.
- Jaagsiekte sheep retrovirus
- , Partial gag gene
- , Clone
- , Sequencing analysis.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain

Corresponding author: LIU Shu-ying,

1. 1. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China
2. College of life science, Northeast Agricultural University, Harbin 150030, China

Abstract: In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, a pair of primers was designed according to the GenBank sequence . The fragment of gag gene was obtained by polymerase chain reaction (PCR), and the gene was cloned into PMD-18 T vector and identified by EcoR I and Sal I digestion. The nucleotide and amino acid sequences of NM strain partial gag gene were compared with the counterpart sequeuces of South Africa strain (Accession No. NC-001494) and USA strain (Accession No. AF105220). The nucleotide and amino acid homology of partial gag gene were 83%, 81.5% and 84%, 83%, respectively. This is the first JSRV nucleotide sequence reported in China.