Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene

JIN Feng-mei; LIN Li-ming; LIN Qi-ying*; XIE Lian-hui

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April 2004

JIN Feng-mei, LIN Li-ming, LIN Qi-ying* and XIE Lian-hui. Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene[J]. Virologica Sinica, 2004, 19(2): 146-148.

Citation: JIN Feng-mei, LIN Li-ming, LIN Qi-ying*, XIE Lian-hui. Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene .VIROLOGICA SINICA, 2004, 19(2) : 146-148.

转RGSV-SP基因水稻植株的再生*

1.
福建农林大学植物病毒研究所, 福建福州 350002

摘要

通过农杆菌介导将水稻草矮病毒（Rice grassy stunt virus, RGSV）编码病害特异蛋白基因sp导入台农67及中花6号品种。经过筛选，再生，获得转化植株。PCR及Southern点杂交分析结果初步表明目的基因片段整合到水稻基因组中。RT-PCR Southern点杂交结果表明目的片段已在植株体内进行了转录。
- RGSV
- , sp基因
- , 转化

Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene

1.
Institute of Plant Virology, Fujian Agricultural and Foresrtry University, Fuzhou 350002，China

Abstract

Rice grassy stunt virus sp gene was transformed into rice by Agrobacterium mediated transformation. PCR analysis and Southern blot hybridization by using the genomic DNA of regenerated plants showed that the sp gene was transformed into rice. By using RT-PCR Southern blot hybridization, it was confirmed that the sp gene had been transtripted into mRNA.
- Rice grassy stunt virus
- , sp gene
- , Transformation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Regeneration of Transgenic Rice with the Rice Grassy Stunt Virus sp Gene

1. Institute of Plant Virology, Fujian Agricultural and Foresrtry University, Fuzhou 350002，China

Abstract: Rice grassy stunt virus sp gene was transformed into rice by Agrobacterium mediated transformation. PCR analysis and Southern blot hybridization by using the genomic DNA of regenerated plants showed that the sp gene was transformed into rice. By using RT-PCR Southern blot hybridization, it was confirmed that the sp gene had been transtripted into mRNA.