本研究旨在以HCV为平台，在简化RT-PCR基础上，结合体外转录，建立一种特异、高效、简便的检测血清中HCV RNA的体外转录合成系统。本法扩增终产物为特定极性的ssRNA，其特异性经凝胶电泳和斑点杂交确定；RNA定量分析结果显示本法核酸扩增效率高于巢式PCR近20倍。

The purpose of this study is to establish a new method which HCV RNA can be used to detect sensitively and specifically through efficient single strand RNAs(ssRNAs) amplification. The sera from the chronic Hepatitis C patients were used as specimens. Single step reverse transcription-PCR (SRT-PCR) was performed prospectively, with a designed set of primers which defined the sequence unique to the 5’ noncoding region of the HCV genome, a great deal of specific ssRNAs were synthesized in the presence of T7RNA polymerase and other appropriate conditions within five hours. Comparing with the nested-PCR, the quantity of the products in our method was nearly 20-fold higher and its specificity was verified by the electrophoresis on agarose gel and the dot hybridization with the probe.