LIU Mei-de, ZHAO Tong-yan*, DONG Yan-de and LU Bao-lin. Immunological Co-sedimentation of Dengue 2 Virus’ Receptor on the C6/36 Cells[J]. Virologica Sinica, 2004, 19(3): 217-219.
Citation:
LIU Mei-de, ZHAO Tong-yan*, DONG Yan-de, LU Bao-lin.
Immunological Co-sedimentation of Dengue 2 Virus’ Receptor on the C6/36 Cells .VIROLOGICA SINICA, 2004, 19(3)
: 217-219.
-
摘要
本文利用差速离心方法提纯的登革2型病毒、抗登革病毒单抗、病毒受体、耦合有G蛋白的琼脂糖珠之间特异性结合的作用,利用免疫共沉淀方法从C6/36细胞中分离鉴定出分子量约为35kDa受体蛋白;并用糖蛋白染色方法否定了此蛋白的糖基化特征。
Immunological Co-sedimentation of Dengue 2 Virus’ Receptor on the C6/36 Cells
-
1.
Key laboratory of vector biology and control, Institute and Microbiology of Epidemiology, AMMS, Beijing 100071,China
-
Abstract
In this paper, based on the especial interaction among purificated Dengue 2 virus, monoclon- al antibody against Dengue virus, virus receptor, and Protein-G-Agrose; one non-glyprotein, which molecular weigt is approximately 35kDa, has been identified to be the receptor of Dengue 2 virus on the C6/36 cells by the method of immunological co-sedimentation. The glyprotein nature of this receptor is also denied through glyprotein stain procedure.
-
-
References
-
Proportional views
-
-