For Preparing the Antigen of Rabies Immunoglobulin in Vero Cells

ZHANG Jie; YE Lin-bai; ZENG Ying-chun; YANG Fan; LIU Jing

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June 2004

ZHANG Jie, YE Lin-bai, ZENG Ying-chun, YANG Fan and LIU Jing. For Preparing the Antigen of Rabies Immunoglobulin in Vero Cells[J]. Virologica Sinica, 2004, 19(3): 242-244.

Citation: ZHANG Jie, YE Lin-bai, ZENG Ying-chun, YANG Fan, LIU Jing. For Preparing the Antigen of Rabies Immunoglobulin in Vero Cells .VIROLOGICA SINICA, 2004, 19(3) : 242-244.

用Vero细胞制备马抗狂犬病血清抗原

叶林柏 ^1,2 ,
曾莹春 ^1*,, ,

1.
1. 武汉大学生命科学院病毒研究所，湖北武汉430072
2.
武汉生物制品研究所，湖北武汉430060

通讯作者： 曾莹春, ;

摘要

以Vero细胞为基质制备马抗狂犬病血清用抗原，以期建立有效、经济、简便的抗原制备方法。用含10%马血清营养液对Vero细胞作适应性培养，接种狂犬病毒，以含1%~3%马血清营养液作维持液培养病毒，于第5，8天收获病毒液，经灭活、浓缩、离心等制成抗原。第5，8天收获病毒滴度可稳定在7.0logLD50/ mL以上，灭活抗原具良好的抗原性，用NIH法测定效价达6.0IU/ mL以上，可用作抗原生产抗狂犬病血清。
- Vero细胞
- , 马血清
- , 抗原

For Preparing the Antigen of Rabies Immunoglobulin in Vero Cells

ZHANG Jie ^1,2 ,
YE Lin-bai ^1*,, ,
ZENG Ying-chun ¹ ,
YANG Fan ¹ ,
LIU Jing ¹

1.
1. Institute of virology,Wuhan University,Wuhan 430072,China
2.
Wuhan lnstitute of biological products, Wuhan 430060, China

Corresponding author: YE Lin-bai,

Abstract

To establish an effective, economical and simple technology for preparing rabies viral antigen that can be used to produce horse anti-rabies serum, rabies virus strain was inoculated in vero cells cultured with medium containing 10% horse serum and was propagated with 199 culture medium containing 1%~3% horse serum. Virus were harvested at the fifth and eighth days and used for preparing antigens by concentration, inactivation and centrifugation. The titers of rabies virus in the culture were reached above 7.0 logLD50/mL .The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.
- Vero cells
- , Horse serum
- , Antigen

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

For Preparing the Antigen of Rabies Immunoglobulin in Vero Cells

Corresponding author: YE Lin-bai,

1. 1. Institute of virology,Wuhan University,Wuhan 430072,China
2. Wuhan lnstitute of biological products, Wuhan 430060, China

Abstract: To establish an effective, economical and simple technology for preparing rabies viral antigen that can be used to produce horse anti-rabies serum, rabies virus strain was inoculated in vero cells cultured with medium containing 10% horse serum and was propagated with 199 culture medium containing 1%~3% horse serum. Virus were harvested at the fifth and eighth days and used for preparing antigens by concentration, inactivation and centrifugation. The titers of rabies virus in the culture were reached above 7.0 logLD50/mL .The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.