Comparison of DNA Polymerase Gene of Marek’s Disease Virus among  Different Pathotypes Strains

WANG Zeng-fu; JIANG Shi-jin; CUI Zhi-zhong; Bublot Michel

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June 2004

WANG Zeng-fu, JIANG Shi-jin, CUI Zhi-zhong and Bublot Michel. Comparison of DNA Polymerase Gene of Marek’s Disease Virus among Different Pathotypes Strains[J]. Virologica Sinica, 2004, 19(3): 259-263.

Citation: WANG Zeng-fu, JIANG Shi-jin, CUI Zhi-zhong, Bublot Michel. Comparison of DNA Polymerase Gene of Marek’s Disease Virus among Different Pathotypes Strains .VIROLOGICA SINICA, 2004, 19(3) : 259-263.

不同致病型马立克氏病病毒DNA聚合酶基因序列比较*

王增福 ¹ ,
姜世金 ¹ ,
崔治中 ^1*,, ,

1.
1.山东农业大学动物科技学院，山东泰安 271018
2.
Merial Select, Inc, 29 Ave. Dony, Garnier Lyon, 69007, France

通讯作者： 崔治中, ;

摘要

为了分析马立克氏病病毒（MDV）致病型与其DNA聚合酶基因的关系，本研究比较了9个不同致病型的该基因的同源性关系，这包括四种不同致病型的国际参考株即弱毒疫苗株CVI988/Ripens株、强毒株GA、超强毒株Md5和特超强毒株648A；中国疫苗株814、中国强毒参考株Jing-1及3个中国野毒株。结果表明：MDV的DNA聚合酶基因非常保守，在比较的9个毒株间，该基因上游约369个碱基的调控序列的同源性在96.7%~100%之间，该基因编码的1220个氨基酸序列的同源性在99.2%~100%之间。尽管不同毒株在一些位点上出现了氨基酸的变异，但这些变异与病毒的致病型或地域分布没有明显的关系。
- 致病型
- , MDV
- , DNA聚合酶基因
- , 序列比较

Comparison of DNA Polymerase Gene of Marek’s Disease Virus among Different Pathotypes Strains

1.
1.College of Animal Science and Technology, Shangdong Agricultural University, Tai’an 271018, China
2.
Merial Select, Inc, 29 Ave. Dony, Garnier Lyon, 69007, France

Corresponding author: CUI Zhi-zhong,

Abstract

In this study, the DNA polymerase genes of 9 different pathotypes strains were compared, to analyze the relationship between the pathotype and MDV’s DNA polymerase gene. These strains included four international reference strains—vaccine strain CVI988, virulent strain GA, very virulent strain Md5 and very virulent plus strain 648A; and other vaccine strain 814, reference strain Jing-1 and three field strains isolated in China. The results showed that the DNA polymerase gene was highly conserved. The homology of upstream regulating sequences among 9 different strains was 96.7%~100%. The identity of amino acid sequences of DNA polymerase among 9 MDV strains of different pathotypes was 99.2%~100%. Although there are variations among them, there are no direct relationship between the DNA polymerase gene and pathotype or zone distribution.
- Pathotypes
- , Marek’s disease viruses
- , DNA Polymerase Gene
- , Sequence Comparison

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparison of DNA Polymerase Gene of Marek’s Disease Virus among Different Pathotypes Strains

Corresponding author: CUI Zhi-zhong,

1. 1.College of Animal Science and Technology, Shangdong Agricultural University, Tai’an 271018, China
2. Merial Select, Inc, 29 Ave. Dony, Garnier Lyon, 69007, France

Abstract: In this study, the DNA polymerase genes of 9 different pathotypes strains were compared, to analyze the relationship between the pathotype and MDV’s DNA polymerase gene. These strains included four international reference strains—vaccine strain CVI988, virulent strain GA, very virulent strain Md5 and very virulent plus strain 648A; and other vaccine strain 814, reference strain Jing-1 and three field strains isolated in China. The results showed that the DNA polymerase gene was highly conserved. The homology of upstream regulating sequences among 9 different strains was 96.7%~100%. The identity of amino acid sequences of DNA polymerase among 9 MDV strains of different pathotypes was 99.2%~100%. Although there are variations among them, there are no direct relationship between the DNA polymerase gene and pathotype or zone distribution.