Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo

LU Yu-rong; FENG Zong-di; WU Dong; SUN Xiu-lian; DENG Fei; YUAN Li; HU Zhi-hong

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June 2004

LU Yu-rong, FENG Zong-di, WU Dong, SUN Xiu-lian, DENG Fei, YUAN Li and HU Zhi-hong. Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo[J]. Virologica Sinica, 2004, 19(3): 276-280.

Citation: LU Yu-rong, FENG Zong-di, WU Dong, SUN Xiu-lian, DENG Fei, YUAN Li, HU Zhi-hong. Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo .VIROLOGICA SINICA, 2004, 19(3) : 276-280.

含外源类蜗牛毒素基因的AcMNPV的虫体感染性研究*

卢玉蓉 ¹ ,
冯枞棣 ¹ ,
孙修炼 ¹ ,
胡志红 ¹ ,

1.
1. 中国科学院武汉病毒研究所分子病毒学重点实验室和无脊椎动物病毒学联合开放实验室，湖北武汉 430071
2.
Department of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, the Netherlands

摘要

类蜗牛毒素基因（conotoxinlike, ctl）是在一些杆状病毒基因组中存在的与蜗牛毒素类似的一类基因，其功能尚不清楚。本文利用苜蓿银纹夜蛾核多角病毒（AcMNPV）bacmid表达系统构建了含油桐尺蠖核多角体病毒（BusuNPV）ctl基因的重组病毒AcBac-ph-ctl。在细胞水平上对ctl基因的RT-PCR分析表明，该基因转录出mRNA。在甜菜夜蛾体内进行了生物活性测定，结果表明AcBac-ph-ctl与对照野生型AcMNPV的LC50，ST50无显著性差异，表明在此系统中，外源的CTL无杀虫增效性能。
- 类蜗牛毒素基因（ctl）
- , AcMNPV Bacmid
- , LC50
- , ST50
- , 转录：生物测定

Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo

LU Yu-rong ¹ ,
FENG Zong-di ¹ ,
WU Dong ¹ ,
SUN Xiu-lian ¹ ,
DENG Fei ¹ ,
YUAN Li ¹ ,
HU Zhi-hong ^1*,,

1.
1.The key laboratory of Molecular Virology and Joint-lab of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071 China
2.
Department of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, the Netherlands

Corresponding author: HU Zhi-hong,

Abstract

The conotoxinlike (ctl) gene is a small gene in the genomes of several baculoviruses. The biological function of baculoviral ctl gene remains unknown. In this report, we generated a recombinant AcMNPV(AcBac-ph-ctl) which contains the ctl gene from BusuNPV using AcMNPV bacmid system. The transcription of ctl gene by AcBac-ph-ctl was confirmed by RT-PCR in infected Sf21 cells. To study the CTL function in vivo, the recombinant viruses were used to infect Spodotera exigua peros, and the infectivity and action speed were determined. No significant difference between the median lethal concentration (LC50) and the median survival time (ST50) of the wild type virus AcMNPV and the recombinant virus was found .
- Conotoxinlike（ctl）
- , AcMNPV Bacmid
- , LC50
- , ST50
- , Transcription

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo

Corresponding author: HU Zhi-hong,

1. 1.The key laboratory of Molecular Virology and Joint-lab of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071 China
2. Department of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, the Netherlands

Abstract: The conotoxinlike (ctl) gene is a small gene in the genomes of several baculoviruses. The biological function of baculoviral ctl gene remains unknown. In this report, we generated a recombinant AcMNPV(AcBac-ph-ctl) which contains the ctl gene from BusuNPV using AcMNPV bacmid system. The transcription of ctl gene by AcBac-ph-ctl was confirmed by RT-PCR in infected Sf21 cells. To study the CTL function in vivo, the recombinant viruses were used to infect Spodotera exigua peros, and the infectivity and action speed were determined. No significant difference between the median lethal concentration (LC50) and the median survival time (ST50) of the wild type virus AcMNPV and the recombinant virus was found .