Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP

DENG Yu-xiu; JIANG Ping; LI Yu-feng; WANG Xian-wei; JIANG Wen-ming; TANG Jing-yuan

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August 2004

DENG Yu-xiu, JIANG Ping, LI Yu-feng, WANG Xian-wei, JIANG Wen-ming and TANG Jing-yuan. Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP[J]. Virologica Sinica, 2004, 19(4): 398-400.

Citation: DENG Yu-xiu, JIANG Ping, LI Yu-feng, WANG Xian-wei, JIANG Wen-ming, TANG Jing-yuan. Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP .VIROLOGICA SINICA, 2004, 19(4) : 398-400.

猪繁殖与呼吸综合征病毒华东地区分离株RT-PCR-RFLP分析*

1.
南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京, 210095

通讯作者： 姜平*, ;

摘要
- 猪繁殖与呼吸综合征病毒
- , RT-PCR
- , 限制性片段长度多态性

Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP

1.
Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agriculture University, Nanjing 210095,China

Corresponding author: JIANG Ping,

Abstract

Porcine reproductive and respiratory syndrome virus(PRRSV) isolates identified in samples from 50 field cases originated from eastern China herds were studied. ORF5 gene of PRRSV isolates was amplified by reverse transcript polymerase chain reaction(RT-PCR) and restriction fragment length polymorphism (RFLP) patterns for enzymes MluⅠ, HincⅡand SacⅡwere determined on these ORF5 genes. The RFLP pattern obtained from 39 isolates was 2-2-1(78%), 3 isolates was 1-1-1(6%) and 8 isolates identified in 2003 was 1-3-1 (16%). The results showed PRRSV strains have variations and existed no less than 3 gene subtypes in the area.
- PRRSV
- , RT-PCR
- , RFLP

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP

Corresponding author: JIANG Ping,

1. Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agriculture University, Nanjing 210095,China

Abstract: Porcine reproductive and respiratory syndrome virus(PRRSV) isolates identified in samples from 50 field cases originated from eastern China herds were studied. ORF5 gene of PRRSV isolates was amplified by reverse transcript polymerase chain reaction(RT-PCR) and restriction fragment length polymorphism (RFLP) patterns for enzymes MluⅠ, HincⅡand SacⅡwere determined on these ORF5 genes. The RFLP pattern obtained from 39 isolates was 2-2-1(78%), 3 isolates was 1-1-1(6%) and 8 isolates identified in 2003 was 1-3-1 (16%). The results showed PRRSV strains have variations and existed no less than 3 gene subtypes in the area.