Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and  Respiratory Syndrome Virus

XU Li-hua; SU Xin-ming; LIU Hua-lei; REN Xue-feng; WANG Zhi-liang; WU Yan-gong; CHEN Pu-yan

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August 2004

XU Li-hua, SU Xin-ming, LIU Hua-lei, REN Xue-feng, WANG Zhi-liang, WU Yan-gong and CHEN Pu-yan. Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus[J]. Virologica Sinica, 2004, 19(4): 401-403.

Citation: XU Li-hua, SU Xin-ming, LIU Hua-lei, REN Xue-feng, WANG Zhi-liang, WU Yan-gong, CHEN Pu-yan. Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus .VIROLOGICA SINICA, 2004, 19(4) : 401-403.

检测猪繁殖与呼吸综合征抗体的重组N蛋白—乳胶凝集试验的建立*

许立华 ^1,2 ,
苏鑫铭 ¹ ,
刘华雷 ¹ ,
任雪枫 ¹ ,
王志亮 ³ ,
吴延功 ³ ,
陈溥言 ^1*,,

1.
1. 南京农业大学农业部畜禽疫病诊断与免疫重点开放实验室，江苏南京210095
2.
宁夏大学农学院动物科学系，宁夏银川 750021
3.
农业部动物检疫所，山东青岛 266032

通讯作者： 陈溥言,

摘要
- 重组N蛋白
- , 猪繁殖与呼吸综合征抗体
- , 血清抗体
- , 乳胶凝集试验

Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus

1.
1. Key Laboratory of Animal Disease Diagnosis and Immunology Nanjing Agricultural University，Nanjing 210095，China
2.
Department of Animal Science,Agricultural College of Ningxia University，Yinchuan 750021，China
3.
Animal Quarantine Institute of Agriculture Ministry，QingDao 266032，China

Corresponding author: CHEN Pu-yan,

Abstract

The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration，temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method’s sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%, 78%, respectively.
- Recombinant N protein
- , Antibodies of porcine reproductive and respiratory syndrome
- , Serum antibody
- , Latex agglutination test

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus

Corresponding author: CHEN Pu-yan,

1. 1. Key Laboratory of Animal Disease Diagnosis and Immunology Nanjing Agricultural University，Nanjing 210095，China
2. Department of Animal Science,Agricultural College of Ningxia University，Yinchuan 750021，China
3. Animal Quarantine Institute of Agriculture Ministry，QingDao 266032，China

Abstract: The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration，temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method’s sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%, 78%, respectively.