The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection

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August 2004

The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection[J]. Virologica Sinica, 2004, 19(4): 404-406.

Citation: . The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection .VIROLOGICA SINICA, 2004, 19(4) : 404-406.

猪瘟病毒C株兔脾毒在SK6细胞中的增殖培养及其鉴定*

张淼涛 ^1,2 ,
刘湘涛 ^1* ,
张彦明 ² ,
谢庆阁 ¹

1.
1. 中国农业科学院兰州兽医研究所畜禽疫病重点实验室，甘肃兰州 730046
2.
西北农林科技大学, 陕西杨凌, 710021

摘要
- 猪瘟病毒(CSFV)
- , C株
- , 增殖培养
- , 鉴定

The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection

^1,2 ,
^1* ,
² ,
¹

1.
1. Lanzhou Veterinary Research Institute, Lanzhou 730046, China
2.
North-West Sci-Tech University of Agriculture and Forestry, Yanling 710021, China

Abstract

Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower level. Direct florescent antibody staining method was not suitable for the detection of attenuated lapinized C-strain. The study provided a primary guide for the detection of attenuated classical swine fever virus. It also supplies an elementary foundation for the study of its growth characteristic in SK6.
- Classical swine fever virus
- , C-strain
- , Multiplication culture
- , Detection.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection

^1,2 ,
^1* ,
² ,
¹

1. 1. Lanzhou Veterinary Research Institute, Lanzhou 730046, China
2. North-West Sci-Tech University of Agriculture and Forestry, Yanling 710021, China

Abstract: Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower level. Direct florescent antibody staining method was not suitable for the detection of attenuated lapinized C-strain. The study provided a primary guide for the detection of attenuated classical swine fever virus. It also supplies an elementary foundation for the study of its growth characteristic in SK6.