Study on the Different Expression Proteins of MT4 Cells Infected by HIV-1 ⅢB Strain

LI Lin; YING Tian-yi; ZHUANG Dao-min; BAO Zuo-yi; LIU Si-yang; LI Han-ping; LIU Yong-jian; YANG Kun; LI Jue; WANG Heng-liang; HUANG Liu-yu; LI Jing-yun

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October 2004

LI Lin, YING Tian-yi, ZHUANG Dao-min, BAO Zuo-yi, LIU Si-yang, LI Han-ping, LIU Yong-jian, YANG Kun, LI Jue, WANG Heng-liang, HUANG Liu-yu and LI Jing-yun. Study on the Different Expression Proteins of MT4 Cells Infected by HIV-1 ⅢB Strain[J]. Virologica Sinica, 2004, 19(5): 435-438.

Citation: LI Lin, YING Tian-yi, ZHUANG Dao-min, BAO Zuo-yi, LIU Si-yang, LI Han-ping, LIU Yong-jian, YANG Kun, LI Jue, WANG Heng-liang, HUANG Liu-yu, LI Jing-yun. Study on the Different Expression Proteins of MT4 Cells Infected by HIV-1 ⅢB Strain .VIROLOGICA SINICA, 2004, 19(5) : 435-438.

HIV感染导致MT4细胞蛋白表达差异的初步研究

应天翼 ¹ ,
庄道民 ² ,
鲍作义 ¹ ,
刘思扬 ¹ ,
李韩平 ¹ ,
刘永健 ¹ ,
王恒良 ¹ ,
黄留玉 ¹ ,
李敬云 ¹ ,

1.
1.军事医学科学院微生物流行病研究所全军艾滋病检测中心，北京100071
2.
军事医学科学院生物工程研究所，北京100071

摘要

为了比较MT4细胞株感染HIV-1的ⅢB株前后的蛋白质表达差异，我们分别提取MT4细胞及感染了人类免疫缺陷病毒(HIV)的MT4细胞的总蛋白质，通过双向电泳分离，使用Image Master 2D Elite 3.10图像分析软件分析获得的凝胶图谱，寻找差异点，使用质谱仪鉴定获得的差异点蛋白质。结果表明感染HIV和未感染HIV的MT4细胞有40个蛋白质点差异，HIV感染后减少的蛋白质点有12个，增多的有28个，通过质谱分析，29个蛋白质得到鉴定。其中HIV感染后下调的蛋白质有能量代谢相关蛋白、肌动蛋白相关蛋白及假想蛋白等；上调的蛋白有肌动蛋白、酶类蛋白、免疫蛋白及假想蛋白等。通过研究我们可以看出宿主细胞感染HIV病毒后有多个蛋白发生变化，可能和HIV与宿主细胞的相互作用有关。为了研究HIV感染的机制必须去除高丰度蛋白，针对特定功能的蛋白质进行具体研究。
- MT4
- , 人免疫缺陷病毒（Human immunodeficiency virus, HIV）
- , 双向电泳
- , 肽质量指文谱分析

Study on the Different Expression Proteins of MT4 Cells Infected by HIV-1 ⅢB Strain

1.
1. Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
2.
Institute of Biotechnology, Academy of Military Medical Science, Beijing 100071, China

Corresponding author: LI Jing-yun,

Abstract

Abstract：To analyze the diferent expression proteins of MT4 cells infected witl1 HIV 1 IIIB strain．The proteins of MT4 cells from infected and Uninfected HIV 1 IIIB were separated bv 2DE and the gels were an alyzed with software Image Master 2D Elite 3．01 to find out the diferent spots．The proteins in the spots were identified by matrix associated laser desorption／ionization time．of-flight(MALDI．TOF) mass spectrometry．A total of 40 diferent protein spots were found．1 2 proteins were down-regulated in the M T4 cells after infection；the other 28 proteins were up．regulated．29 proteins were detected bv M ALDI—T0E Th e proteins that down．regulated in MT4 cells after infecteion include proteins related to energy metabolism，actin proteins an d hypothetical proteins．The up—regulated proteins in MT4 cells after infecteion include proteins related to actin proteins，enzymes．immune proteins an d hypothetical proteins．In the M T4 cells infected bv HI man y proteins were chan ged．In order to understand the mechanism of infection of HIV,we must wipe Of the abundan t proteins an d study the proteins witl1 special functions．
- MT4
- , HIV
- , Two-dimensional electrophoresis
- , Peptide mass fingerprinting

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on the Different Expression Proteins of MT4 Cells Infected by HIV-1 ⅢB Strain

Corresponding author: LI Jing-yun,

1. 1. Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
2. Institute of Biotechnology, Academy of Military Medical Science, Beijing 100071, China

Abstract: Abstract：To analyze the diferent expression proteins of MT4 cells infected witl1 HIV 1 IIIB strain．The proteins of MT4 cells from infected and Uninfected HIV 1 IIIB were separated bv 2DE and the gels were an alyzed with software Image Master 2D Elite 3．01 to find out the diferent spots．The proteins in the spots were identified by matrix associated laser desorption／ionization time．of-flight(MALDI．TOF) mass spectrometry．A total of 40 diferent protein spots were found．1 2 proteins were down-regulated in the M T4 cells after infection；the other 28 proteins were up．regulated．29 proteins were detected bv M ALDI—T0E Th e proteins that down．regulated in MT4 cells after infecteion include proteins related to energy metabolism，actin proteins an d hypothetical proteins．The up—regulated proteins in MT4 cells after infecteion include proteins related to actin proteins，enzymes．immune proteins an d hypothetical proteins．In the M T4 cells infected bv HI man y proteins were chan ged．In order to understand the mechanism of infection of HIV,we must wipe Of the abundan t proteins an d study the proteins witl1 special functions．