YIN Hong-yan, DENG Gang, SHA Ri-na, QIAO Wen-tao, GENG Yun-qi and CHEN Qi-min*. Primary Study on the Interaction of JDV and Other Three Bovine Retroviruses[J]. Virologica Sinica, 2004, 19(5): 471-475.
Citation: YIN Hong-yan, DENG Gang, SHA Ri-na, QIAO Wen-tao, GENG Yun-qi, CHEN Qi-min*. Primary Study on the Interaction of JDV and Other Three Bovine Retroviruses .VIROLOGICA SINICA, 2004, 19(5) : 471-475.

JDV与三种牛反转录病毒相互关系的初步研究*

  • 为研究JDV与其它三种牛反转录病毒BIV、BLV、BFV的相互作用关系,将以JDV、BIV、BLV、BFV的 LTR为启动子,以Luc为报告基因的质粒和以上病毒反式激活因子的表达质粒共转染BL12细胞系,通过瞬时表达分析试验证明了JDV和BIV的LTR和Tat之间亲缘关系很近,能够相互激活;JDV Tat可以反式激活BLV LTR,BLV Tax不能激活JDV LTR;JDV LTR上存在BFV Tas的应答元件;BLV、BFV和BIV的LTR和反式激活因子间不存在相互激活。

Primary Study on the Interaction of JDV and Other Three Bovine Retroviruses

  • To study the interaction among Jembrana disease virus, Bovine immunodeficiency virus, Bovine Foamy Virus and Bovine Leukemia Virus, a series of plasmids were constructed with their LTR and luc report gene, respectively, and transactivator expression plasmids of these four viruses were also constructed. These plasmids were co-transfected into BL12 cell line, respectively. Transient assay showed the interactivation between LTR and Tat of BIV and JDV, suggesting that they could be transactivated each other. In addition, BFV Tas could transactivate JDV LTR and JDV Tat could transactivate BLV LTR whereas BLV Tax had no effect on JDV LTR and no other interactivation was detected among BLV, BFV and BIV.

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    Primary Study on the Interaction of JDV and Other Three Bovine Retroviruses

    • 1. College of Life Science, Nankai University, Tianjin 300071, China

    Abstract: To study the interaction among Jembrana disease virus, Bovine immunodeficiency virus, Bovine Foamy Virus and Bovine Leukemia Virus, a series of plasmids were constructed with their LTR and luc report gene, respectively, and transactivator expression plasmids of these four viruses were also constructed. These plasmids were co-transfected into BL12 cell line, respectively. Transient assay showed the interactivation between LTR and Tat of BIV and JDV, suggesting that they could be transactivated each other. In addition, BFV Tas could transactivate JDV LTR and JDV Tat could transactivate BLV LTR whereas BLV Tax had no effect on JDV LTR and no other interactivation was detected among BLV, BFV and BIV.

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