LI Zi-jian, JIN Ning-yi, ZHANG Li-su, JIANG Wen-zheng and ZHANG Hong-yong. Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c[J]. Virologica Sinica, 2004, 19(6): 559-562.
Citation:
LI Zi-jian, JIN Ning-yi, ZHANG Li-su, JIANG Wen-zheng, ZHANG Hong-yong.
Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c .VIROLOGICA SINICA, 2004, 19(6)
: 559-562.
HIV-2 gag-gp105嵌合基因DNA疫苗对小鼠的免疫原性研究
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李子健
2
,
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金宁一
1*,,
,
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张立树
1
,
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江文正
1
,
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张洪勇
1
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摘要
利用真核表达载体pVAX1构建HIV-2 gag-gp105嵌合基因的重组质粒pVAX1gag-gp105,将其转入BHK21细胞中,利用间接免疫荧光方法检测其表达情况。进一步分别将核酸疫苗质粒pVAX1gag-gp105、对照组质粒pVAX1和PBS溶液经肌肉注射免疫BALB/c小鼠,检测免疫小鼠脾CD4+、CD8+T细胞亚群的数量,脾特异性CTL 杀伤活性和血清抗体滴度。结果显示,重组核酸疫苗质粒pVAX1gag-gp105疫组小鼠脾CD4+、CD8+T细胞亚群的数值均比对照组高( P0.01),脾特异性CTL 杀伤活性与对照组相比差异极显著(P0.01),血清抗体滴度显著高于对照组(P0.01) 。以上结果表明,HIV-2 gag-gp105嵌合基因DNA疫苗对BALB/c小鼠具有良好的体液和细胞免疫原性。
Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c
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1.
1. Key Laboratory of Genetic Engineering of PLA, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, China
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Corresponding author:
JIN Ning-yi,
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Abstract
The recombinant expression plasmid of pVAX1gag-gp105 was constructed and transfected into BHK21 cells by lipofectamine, the expressed product was detected by indirect immunofluore scence. After the mice were immunized with pVAX1gag-gp105 and control plasmid, the number of CD4+and CD8+ subgroups of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Compared with vaccinated control groups, pVAX1gag-gp105 vaccinated group had significant differences in the titers of the serum antibodies (P0.01), the numbers of spleen CD4+ and CD8+ T cells (P0.05) and the specific killing activities of spleen CTL (P0.01). These results manifested that the DNA vaccine based on HIV-2 chimeric gene gag-gp105 possessed good humoral and cellular immunogenecity in BALB/c mice.
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References
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Proportional views
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