LI Zi-jian, JIN Ning-yi, ZHANG Li-su, JIANG Wen-zheng and ZHANG Hong-yong. Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c[J]. Virologica Sinica, 2004, 19(6): 559-562.
Citation: LI Zi-jian, JIN Ning-yi, ZHANG Li-su, JIANG Wen-zheng, ZHANG Hong-yong. Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c .VIROLOGICA SINICA, 2004, 19(6) : 559-562.

HIV-2 gag-gp105嵌合基因DNA疫苗对小鼠的免疫原性研究

  • 通讯作者: 金宁一, 
  • 利用真核表达载体pVAX1构建HIV-2 gag-gp105嵌合基因的重组质粒pVAX1gag-gp105,将其转入BHK21细胞中,利用间接免疫荧光方法检测其表达情况。进一步分别将核酸疫苗质粒pVAX1gag-gp105、对照组质粒pVAX1和PBS溶液经肌肉注射免疫BALB/c小鼠,检测免疫小鼠脾CD4+、CD8+T细胞亚群的数量,脾特异性CTL 杀伤活性和血清抗体滴度。结果显示,重组核酸疫苗质粒pVAX1gag-gp105疫组小鼠脾CD4+、CD8+T细胞亚群的数值均比对照组高( P0.01),脾特异性CTL 杀伤活性与对照组相比差异极显著(P0.01),血清抗体滴度显著高于对照组(P0.01) 。以上结果表明,HIV-2 gag-gp105嵌合基因DNA疫苗对BALB/c小鼠具有良好的体液和细胞免疫原性。

Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c

  • Corresponding author: JIN Ning-yi, 
  • The recombinant expression plasmid of pVAX1gag-gp105 was constructed and transfected into BHK21 cells by lipofectamine, the expressed product was detected by indirect immunofluore scence. After the mice were immunized with pVAX1gag-gp105 and control plasmid, the number of CD4+and CD8+ subgroups of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Compared with vaccinated control groups, pVAX1gag-gp105 vaccinated group had significant differences in the titers of the serum antibodies (P0.01), the numbers of spleen CD4+ and CD8+ T cells (P0.05) and the specific killing activities of spleen CTL (P0.01). These results manifested that the DNA vaccine based on HIV-2 chimeric gene gag-gp105 possessed good humoral and cellular immunogenecity in BALB/c mice.

  • 加载中
  • 加载中

Article Metrics

Article views(3984) PDF downloads(994) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c

      Corresponding author: JIN Ning-yi,
    • 1. 1. Key Laboratory of Genetic Engineering of PLA, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, China

    Abstract: The recombinant expression plasmid of pVAX1gag-gp105 was constructed and transfected into BHK21 cells by lipofectamine, the expressed product was detected by indirect immunofluore scence. After the mice were immunized with pVAX1gag-gp105 and control plasmid, the number of CD4+and CD8+ subgroups of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Compared with vaccinated control groups, pVAX1gag-gp105 vaccinated group had significant differences in the titers of the serum antibodies (P0.01), the numbers of spleen CD4+ and CD8+ T cells (P0.05) and the specific killing activities of spleen CTL (P0.01). These results manifested that the DNA vaccine based on HIV-2 chimeric gene gag-gp105 possessed good humoral and cellular immunogenecity in BALB/c mice.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return