LIU Yan-hua, FAN Wei-xing, SUI Zhao-feng, WANG Xi-le, XU Long-tao, SUN Zhong-cheng and GAO Bao-shan. Cloning and Sequence Analysis of vp2 Gene of Porcine Parvovirus SD-68 Strain[J]. Virologica Sinica, 2004, 19(6): 568-571.
Citation: LIU Yan-hua, FAN Wei-xing, SUI Zhao-feng, WANG Xi-le, XU Long-tao, SUN Zhong-cheng, GAO Bao-shan. Cloning and Sequence Analysis of vp2 Gene of Porcine Parvovirus SD-68 Strain .VIROLOGICA SINICA, 2004, 19(6) : 568-571.

猪细小病毒SD-68株vp2基因的克隆及序列分析

  • 通讯作者: 范伟兴, 
  • 对猪细小病毒(PPV)SD-68株vp2基因进行的克隆和序列测定表明:SD-68株VP2基因全长1740bp,编码579个氨基酸残基组成的多肽;PPV SD-68株与Kresse株、SY-99株、NADL-2(5075)株、NADL-2(4973)株、US-1株的VP2基因比较,核苷酸的同源性在99%以上,氨基酸的同源性在96%以上。进化树分析表明SD-68株与kresse株的亲缘关系最近;在决定毒株组织嗜性的关键氨基酸位点上(378,383及436),SD-68株与kresse株的差异最小,据此推测SD-68株的组织嗜性与Kresse株相似,即SD-68株属皮炎型PPV; 而比较弱毒株NADL-2、SD-68和强毒株kresse VP2的氨基酸差异后发现,215、378和383可能是决定PPV致病性强弱的关键位点。

Cloning and Sequence Analysis of vp2 Gene of Porcine Parvovirus SD-68 Strain

  • Corresponding author: FAN Wei-xing, 
  • vp2 gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced .The results showed that vp2 gene of Porcine parvovirus (PPV) SD-68 strain was 1740bp and encoded a protein of 579 amino acids. vp2 gene of SD-68 strain exhibited over 99% nucleotide sequence identity and 96% amino acid sequence identity with other PPV strains: Kresse, SY-99, NADL-2(5075), NADL-2(4973), US-1. Phylogenetic tree analysis of vp2 gene showed the closest relationship between SD-68 strain and kresse strain. The difference between SD-68 strain and kresse strain was only on 378, 383 and 436 residues, which decided the tissue tropism of PPV, so the tissue tropism of SD-68 strain suggested more similar with kresse stain, that is to say, SD-68 was a dermatitis strain. Comparison of amino acid residues of virulent strain (kreese) and vaccine strain (NADL-2 and SD-68) suggested that 215, 378 and 383 were important residues that decided the pathogenicity of PPV.

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    Cloning and Sequence Analysis of vp2 Gene of Porcine Parvovirus SD-68 Strain

      Corresponding author: FAN Wei-xing,
    • 1. 1.College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
    • 2. China Animal Epizootiology Center, Qingdao 266032, China
    • 3. Qilu Animal Health Products Factory, Jinan 250001, China

    Abstract: vp2 gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced .The results showed that vp2 gene of Porcine parvovirus (PPV) SD-68 strain was 1740bp and encoded a protein of 579 amino acids. vp2 gene of SD-68 strain exhibited over 99% nucleotide sequence identity and 96% amino acid sequence identity with other PPV strains: Kresse, SY-99, NADL-2(5075), NADL-2(4973), US-1. Phylogenetic tree analysis of vp2 gene showed the closest relationship between SD-68 strain and kresse strain. The difference between SD-68 strain and kresse strain was only on 378, 383 and 436 residues, which decided the tissue tropism of PPV, so the tissue tropism of SD-68 strain suggested more similar with kresse stain, that is to say, SD-68 was a dermatitis strain. Comparison of amino acid residues of virulent strain (kreese) and vaccine strain (NADL-2 and SD-68) suggested that 215, 378 and 383 were important residues that decided the pathogenicity of PPV.

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