YAN Xiao, LI Tian-xian*, FAN Zhao-jun, KOU Zheng and CHEN Sheng-liang. Sequencing and Phelogenetic Analysis of Hypervariable Region of Infectious Bursal Disease Virus vp2 Gene[J]. Virologica Sinica, 2004, 19(6): 620-623.
Citation: YAN Xiao, LI Tian-xian*, FAN Zhao-jun, KOU Zheng, CHEN Sheng-liang. Sequencing and Phelogenetic Analysis of Hypervariable Region of Infectious Bursal Disease Virus vp2 Gene .VIROLOGICA SINICA, 2004, 19(6) : 620-623.

IBDVvp2基因高变区序列测定与进化分析

  • 本实验采用来源于江苏地区的六株不同鸡传染性法氏囊病病毒(Infectious bursal disease virus,IBDV)毒株,应用RT-PCR法对vp2基因高变区进行了扩增,构建重组质粒pMD18T- vp2,测序。与有代表性的 IBDV毒株VP2基因高变区序列进行比较分析,以ClustalX软件进行序列比对,得到基因序列及氨基酸序列同源性,IBDV Y3、P2G、P8G、SZ、Y5 和W04(6株)IBDV与D6984(荷兰)的同源性达到99.0%以上,与其它一些超强毒株(very virulent IBDV,vvIBDV)的同源性也达到了97.8%以上,并在关键氨基酸位点符合vvIBDV特征,采用Phylip3.5软件分析作出进化树,其结果从分子水平说明六个毒株均为vvIBDV,与欧洲和日本的超强毒株有较近的亲缘关系,而与美洲株的较远,从而为IBDV分子流行病学研究和疫苗的研制提供了科学依据。

Sequencing and Phelogenetic Analysis of Hypervariable Region of Infectious Bursal Disease Virus vp2 Gene

  • Hypervariable region of vp2 gene of six different IBDV (Infectious bursal disease virus) isolates, which were isolated from Jiangsu province, was amplified by RT-PCR and sequenced. Furthermore, sequences of six IBDV isolates were analyzed by ClustalX and Phylip3.5. It was suggested that three isolates (Y3, P2G, P8G) were closely related to vvIBDV (very virulent IBDV) strains isolated from China and Europe. Other isolates (SZ, Y5, W04) were very close with Japanese vvIBDV strains. This study laid on foundation for the research of molecular epidemiology and the development of IBDV vaccines.

  • 加载中
  • 加载中

Article Metrics

Article views(4338) PDF downloads(828) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Sequencing and Phelogenetic Analysis of Hypervariable Region of Infectious Bursal Disease Virus vp2 Gene

    • 1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    Abstract: Hypervariable region of vp2 gene of six different IBDV (Infectious bursal disease virus) isolates, which were isolated from Jiangsu province, was amplified by RT-PCR and sequenced. Furthermore, sequences of six IBDV isolates were analyzed by ClustalX and Phylip3.5. It was suggested that three isolates (Y3, P2G, P8G) were closely related to vvIBDV (very virulent IBDV) strains isolated from China and Europe. Other isolates (SZ, Y5, W04) were very close with Japanese vvIBDV strains. This study laid on foundation for the research of molecular epidemiology and the development of IBDV vaccines.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return