cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus

DUAN Bing; ZHAO Shu-ling; ZHANG Hai-yuan; ZHANG Xiao-xia; XIAO Yu-zhou; TANG Xian-chun; PENG Hui-yin

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December 2004

DUAN Bing, ZHAO Shu-ling, ZHANG Hai-yuan, ZHANG Xiao-xia, XIAO Yu-zhou, TANG Xian-chun and PENG Hui-yin. cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus[J]. Virologica Sinica, 2004, 19(6): 627-631.

Citation: DUAN Bing, ZHAO Shu-ling, ZHANG Hai-yuan, ZHANG Xiao-xia, XIAO Yu-zhou, TANG Xian-chun, PENG Hui-yin. cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus .VIROLOGICA SINICA, 2004, 19(6) : 627-631.

文山松毛虫质型多角体病毒S8片段cDNA克隆与原核表达

赵淑玲 ^1,2 ,
张海元 ¹ ,
张小霞 ¹ ,
肖宇宙 ¹ ,
汤显春 ¹ ,
彭辉银 ¹ ,

1.
1. 中国科学院武汉病毒研究所分子病毒学重点实验室，湖北武汉 430071
2.
中国科学院研究生院, 北京100039

摘要

文山松毛虫质型多角体病毒(DpwCPV)S8片段被克隆和测序，该片段全长1332bp，编码390个氨基酸组成的分子量大约为43kDa的蛋白P44。根据本实验室测定出的马尾松毛虫质型多角体病毒(DpCPV )基因组全序列,设计引物，扩增出文山松毛虫质型多角体病毒S8部分片段，并亚克隆出p44基因序列，然后将p44基因序列cDNA克隆到表达载体pET-28a中，构建成表达质粒pET-S8，用IPTG诱导大肠杆菌BL21，经SDS-PAGE证明p44基因在大肠杆菌中获得成功表达，并对其编码蛋白序列进行了分析。
- CPV
- , 基因组分析
- , 原核表达
- , p44基因
- , 双链RNA病毒

cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus

1.
1. Key Lab of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences，Wuhan 430071,China
2.
The Graduate School of Chinese Academy of Sciences, Bejing 100039, China

Corresponding author: PENG Hui-yin,

Abstract

The nucleotide sequence of Dendrolimus punctatus Wenshanensis cypovirus genomic segment 8 (S8) has been determined. S8 consists 1332 nucleotides and encodes a putative protein of 390 amino acids with a molecular mass of approximately 44kDa (P44). Then, the fragment containing p44 gene was inserted into pET-28a expression vector and expressed in E.coli BL21. SDS-PAGE results showed that p44 gene has expressed.
- Dendrolimus punctatus Wenshanensis cypovirus
- , Genome analysis
- , p44 gene
- , Prokaryotic expression
- , dsRNA virus.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus

Corresponding author: PENG Hui-yin,

1. 1. Key Lab of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences，Wuhan 430071,China
2. The Graduate School of Chinese Academy of Sciences, Bejing 100039, China

Abstract: The nucleotide sequence of Dendrolimus punctatus Wenshanensis cypovirus genomic segment 8 (S8) has been determined. S8 consists 1332 nucleotides and encodes a putative protein of 390 amino acids with a molecular mass of approximately 44kDa (P44). Then, the fragment containing p44 gene was inserted into pET-28a expression vector and expressed in E.coli BL21. SDS-PAGE results showed that p44 gene has expressed.