WANG Fu-xiang, SUN Yong-tao, LIU Juan, MA Ying-ji, YANG Bao-shan and YAN Bing-zhu. Interleukin-12 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine[J]. Virologica Sinica, 2005, 20(1): 16-19.
Citation: WANG Fu-xiang, SUN Yong-tao, LIU Juan, MA Ying-ji, YANG Bao-shan, YAN Bing-zhu. Interleukin-12 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine .VIROLOGICA SINICA, 2005, 20(1) : 16-19.

IL-12基因对HIV-1核酸疫苗诱导的免疫应答的影响

  • 研究白细胞介素12(IL 12)基因对HIV 1核酸疫苗诱导免疫应答的影响,以探求治疗性HIV 1 核酸疫苗的新策略。将pCI neoGAG联合白细胞介素12基因或者pCI neoGAG单独免疫Balb/c小鼠,通过ELISA检测免疫小鼠的特异性抗体和IFN γ,通过MTT实验检测免疫小鼠脾淋巴细胞增殖实验,通过乳酸脱氢酶(LDH)实验检测小鼠特异性细胞毒性T淋巴细胞(CTL)反应。与pCI neoGAG免疫组比较,pCI neoGAG联合白细胞介素12基因免疫组小鼠血清的抗HIV 1p24 抗体滴度降低,有显著性差异(P 0. 01);而与pCI neoGAG 免疫组比较, pCI neoGAG联合白细胞介素12基因免疫组小鼠血清的IFN γ升高,有显著性差异(P0.01);pCI neoGAG联合白细胞介素12基因免疫组小鼠的脾淋巴细胞增殖实验刺激指数(SI)以及特异性CTL活性均高于pCI neoGAG免疫组,有显著性差异(P0.01)。因此,白细胞介素12基因基因联合HIV 1核酸疫苗免疫小鼠,可能增强特异性Th1细胞和CTL反应,白细胞介素12基因对体液免疫有抑制作用。

Interleukin-12 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine

  • To investigate the effect of interleukin-12 DNA immunization on immune responses induced by HIV-1 nucleic acid vaccine(DNA vaccine)and to explore new strategies for therapeutic HIV-1 DNA vaccine, Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for interleukin-12 (IL-12).Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA , and splenocytes were isolated for detecting antigen-specific lymphoproliferative responses and specific CTL response by MTT assay and LDH assay respectively.Our research showed that the anti-HIV antibody titers of mice co-immunized with pCI-neoGAG and the DNA encoding for interleukin-12 (IL-12) were lower than that of mice immunized with pCI-neoGAG alone(P0.01); In contrast ,the IFN-γ level of mice co-immunized with pCI-neoGAG and the DNA encoding for interleukin-12 was higher than that of mice immunized with pCI-neoGAG alone(P0.01).Furthermore, compared with mice injected with pCI-neoGAG alone, the specific CTL cytotoxity

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    Interleukin-12 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine

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    Abstract: To investigate the effect of interleukin-12 DNA immunization on immune responses induced by HIV-1 nucleic acid vaccine(DNA vaccine)and to explore new strategies for therapeutic HIV-1 DNA vaccine, Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for interleukin-12 (IL-12).Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA , and splenocytes were isolated for detecting antigen-specific lymphoproliferative responses and specific CTL response by MTT assay and LDH assay respectively.Our research showed that the anti-HIV antibody titers of mice co-immunized with pCI-neoGAG and the DNA encoding for interleukin-12 (IL-12) were lower than that of mice immunized with pCI-neoGAG alone(P0.01); In contrast ,the IFN-γ level of mice co-immunized with pCI-neoGAG and the DNA encoding for interleukin-12 was higher than that of mice immunized with pCI-neoGAG alone(P0.01).Furthermore, compared with mice injected with pCI-neoGAG alone, the specific CTL cytotoxity

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