KONG Xian-gang, LI Gui-xia, LIU Sheng-wang and RAN Duo-liang. Analysis of Molecular Characteristics of Goose Parvovirus Strain HG5/82[J]. Virologica Sinica, 2005, 20(1): 28-32.
Citation:
KONG Xian-gang, LI Gui-xia, LIU Sheng-wang, RAN Duo-liang.
Analysis of Molecular Characteristics of Goose Parvovirus Strain HG5/82 .VIROLOGICA SINICA, 2005, 20(1)
: 28-32.
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摘要
本研究应用PCR技术扩增得到GPV中国分离株HG5/82的非结构基因与结构基因,片段大小分别约为1.9kb、2.2kb的片段。将该片段分别进行克隆及序列测定,并与GPV 国内外部分已发表的毒株及番鸭细小病毒(Muscovy duck parvovirus,MDPV)的对应序列比较。结果表明:HG5/82 株非结构蛋白(Non structure, NS)基因长为1884bp,编码627个氨基酸。HG5/82株结构蛋白基因长为2199bp,编码732个氨基酸。序列分析结果表明,我国地方分离株与国内外鹅细小病毒相比,ns基因、vp基因均表现出较高的同源性,并且具有共同的分子特征。为进一步研究GPV的基因功能、遗传变化规律及病毒分子致病机理提供了一定的分子基础。结构基因VP3 间变异较小,这是目前GPV只有一个血清型的分子基础,为基因工程苗的研制提供了可行性。HG5/82 与番鸭细小病毒相应序列比较发现,与细小病毒其它成员相比两者具有较近的亲源关系,但这种同源性明显低于鹅细小病毒之间的同源性。
Analysis of Molecular Characteristics of Goose Parvovirus Strain HG5/82
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Abstract
Non-structural and structural genes of Goose parvovirus (GPV) HG5/82 strain were amplified using polymerase chain reaction (PCR) method and cloned into the pMD18-T vector, respectively. The two fragments were sequenced and analyzed with reference GPV strains and Muscovy duck parvovirus(MDPV) strains. The results showed that the GPV ns gene is 1884bp and encoding 627 amino acids. The vp gene of HG5/82 strain is 2199bp and encoding 732 amino acids. The identity of partial VP3 genes between HG5/82 and MDPV is higher than that between GPV and other members of Parvovirinae. These results were also proved by the analysis of the VP3 gene. The high identity between GPV HG5/82 and MDPV indicated that they would be derived from the same ancestor.
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References
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Proportional views
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