Inhibitory Effects of Nitric oxide on the Porcine Parvovirus infection in vitro

WEI Zhan-yong; CUI Bao-an; HUANG Ke-he; WANG Xue-bin; JIN Xi-xin; ZHANG Long-xian

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February 2005

WEI Zhan-yong, CUI Bao-an, HUANG Ke-he, WANG Xue-bin, JIN Xi-xin and ZHANG Long-xian. Inhibitory Effects of Nitric oxide on the Porcine Parvovirus infection in vitro[J]. Virologica Sinica, 2005, 20(1): 33-36.

Citation: WEI Zhan-yong, CUI Bao-an, HUANG Ke-he, WANG Xue-bin, JIN Xi-xin, ZHANG Long-xian. Inhibitory Effects of Nitric oxide on the Porcine Parvovirus infection in vitro .VIROLOGICA SINICA, 2005, 20(1) : 33-36.

一氧化氮对猪细小病毒体外增殖的影响研究

魏战勇 ¹ ,
崔保安 ² ,
黄克和 ^1*,, ,
王学斌 ² ,
金喜新 ² ,
张龙现 ²

1.
1.江苏南京南京农业大学动物医学院 2.河南农业大学农业生物技术重点开放实验室河南郑州

通讯作者： 黄克和, ;

摘要

本文研究了来源于不同前体物的一氧化氮(Nitro oxide, NO)对猪细小病毒(Porcine parvovirus,PPV)体外增殖的影响。结果表明,NO前体物S 硝基N 乙酰青霉胺(SNAP)、L 精氨酸(L Arg)均能够有效地诱导PK 15 细胞产生NO,进而显著地抑制PPV在PK 15 细胞上的复制,其效果与前体物的浓度呈正相关,在浓度为100μmol/L和200μmol/L时,SNAP产生NO的能力与抑制病毒复制的作用要强于L Arg。在病毒感染前6 h和3 h添加SNAP或L Arg对病毒复制的抑制作用比在病毒感染后3 h 和6 h添加的作用强,表明NO的抗病毒作用主要发生在病毒感染的初始阶段。此外,添加具有抑制L Arg产生NO作用的N 硝基L 精氨酸(L NNA)能抵消L Arg体外抗病毒的作用。
- 一氧化氮
- , 猪细小病毒
- , S-硝基-N-乙酰青霉胺
- , L-精氨酸 N-硝基-L-精氨酸

Inhibitory Effects of Nitric oxide on the Porcine Parvovirus infection in vitro

Corresponding author: HUANG Ke-he,

Abstract

Inhibitory effects of nitric oxide (NO) on Porcine parvovirus (PPV) infection on porcine kidney (PK-15) cells were studied in the paper. The results showed that production of NO was effectively induced in cell cultures treated with S-nitroso-N-acetylpenicllamine (SNAP) or L-arginine and replication of PPV was significantly inhibited by the addition of them in a dose-dependent manner.SNAP produced significantly higher amounts of NO and showed higher level of inhibitory effect on PPV replicaton than L-Arg at 100 and 200 μmol/L. Higher level of inhibition of viral production was also obtained when pretreated with SNAP at 6 h and 3 h before infection than added after infection,suggesting that the anti-PPV effects of NO occurred at the initial stages of PPV infection. In addition, the anti-PPV effects of L-arginine could be blocked by N~G-nitro-L-Arginine.
- Nitric oxide
- , Porcine parvovirus
- , S-nitroso-N-acetylpenicllamine
- , L-Arginine

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Inhibitory Effects of Nitric oxide on the Porcine Parvovirus infection in vitro

Corresponding author: HUANG Ke-he,

Abstract: Inhibitory effects of nitric oxide (NO) on Porcine parvovirus (PPV) infection on porcine kidney (PK-15) cells were studied in the paper. The results showed that production of NO was effectively induced in cell cultures treated with S-nitroso-N-acetylpenicllamine (SNAP) or L-arginine and replication of PPV was significantly inhibited by the addition of them in a dose-dependent manner.SNAP produced significantly higher amounts of NO and showed higher level of inhibitory effect on PPV replicaton than L-Arg at 100 and 200 μmol/L. Higher level of inhibition of viral production was also obtained when pretreated with SNAP at 6 h and 3 h before infection than added after infection,suggesting that the anti-PPV effects of NO occurred at the initial stages of PPV infection. In addition, the anti-PPV effects of L-arginine could be blocked by N~G-nitro-L-Arginine.