Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus

ZHU Yi-xin; ZHANG Xiao-guang; YANG Yi-shu; LIU Chang; QIAO Wen-tao; CHEN Qi-min; GENG Yun-qi

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February 2005

ZHU Yi-xin, ZHANG Xiao-guang, YANG Yi-shu, LIU Chang, QIAO Wen-tao, CHEN Qi-min and GENG Yun-qi. Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus[J]. Virologica Sinica, 2005, 20(1): 37-40.

Citation: ZHU Yi-xin, ZHANG Xiao-guang, YANG Yi-shu, LIU Chang, QIAO Wen-tao, CHEN Qi-min, GENG Yun-qi. Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus .VIROLOGICA SINICA, 2005, 20(1) : 37-40.

牛免疫缺陷病毒基质蛋白和衣壳蛋白的可溶性表达

朱义鑫 ¹ ,
张晓光 ² ,
杨怡姝 ³ ,
刘畅 ¹ ,
乔文涛 ¹ ,
陈启民 ¹ ,
耿运琪 ^1*,,

1.
南开大学生命科学学院天津北京中国疾病预防控制中心病毒病预防控制所北京工业大学生命科学与生物工程学院

通讯作者： 耿运琪,

摘要

基质蛋白和衣壳蛋白是BIV的主要结构蛋白,在病毒感染及整个复制周期中起重要作用。本文采用pTXB系统在大肠杆菌中表达出融合状态的牛免疫缺陷病毒BIV基质蛋白MA及衣壳蛋白CA,经几丁质亲合、自剪切纯化后,获得不含融合片段的纯化产物。每克湿菌体MA产量可达毫克级,CA表达量达十毫克级。用原核表达获得的高纯度CA蛋白免疫大白兔获得的抗血清,经Western Blot 分析显示能够与病毒颗粒的CA蛋白发生特异反应,证实表达产物具有良好的免疫原性和反应原性,可用于制备相应抗体,为研究BIV相应基因表达变化,进行体外蛋白质相互作用试验提供工具。
- 牛免疫缺陷病毒
- , 基质蛋白
- , 衣壳蛋白
- , pTXB表达系统

Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus

Corresponding author: GENG Yun-qi,

Abstract

The matrix and the capsid are main structural proteins of Bovine immunodeficency virus (BIV) and play important roles in virus life cycle. In order to analyze these two proteins in vitro, we cloned these two genes into the prokaryotic expression vector pTXB and expressed in E. coli BL21(DE3) in a soluble form. After purification, the matrix without fusion peptide was obtained in milligram magnitude. The capsid was also expressed and purified in ten-milligram magnitude. We used the dissolvable BIV CA to immunized rabbit and got antiserum 53 d after first injection. Western blotting analysis showed that the antiserum could specifically recognize the native HIV CA protein. The protein could be used as the antigen to produce the antiserum and used in protein-protein interaction analysis.
- BIV
- , Matrix
- , Capsid
- , pTXB system

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus

Corresponding author: GENG Yun-qi,

Abstract: The matrix and the capsid are main structural proteins of Bovine immunodeficency virus (BIV) and play important roles in virus life cycle. In order to analyze these two proteins in vitro, we cloned these two genes into the prokaryotic expression vector pTXB and expressed in E. coli BL21(DE3) in a soluble form. After purification, the matrix without fusion peptide was obtained in milligram magnitude. The capsid was also expressed and purified in ten-milligram magnitude. We used the dissolvable BIV CA to immunized rabbit and got antiserum 53 d after first injection. Western blotting analysis showed that the antiserum could specifically recognize the native HIV CA protein. The protein could be used as the antigen to produce the antiserum and used in protein-protein interaction analysis.