Construction of a Recombinant HaSNPV Expressing GP64

WANG Shu; WANG Hua-lin; DENG Fei; WU Dong; LONG Gang; YUAN Li; HU Zhi-hong

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February 2005

WANG Shu, WANG Hua-lin, DENG Fei, WU Dong, LONG Gang, YUAN Li and HU Zhi-hong. Construction of a Recombinant HaSNPV Expressing GP64[J]. Virologica Sinica, 2005, 20(1): 79-83.

Citation: WANG Shu, WANG Hua-lin, DENG Fei, WU Dong, LONG Gang, YUAN Li, HU Zhi-hong. Construction of a Recombinant HaSNPV Expressing GP64 .VIROLOGICA SINICA, 2005, 20(1) : 79-83.

表达GP64的重组HaSNPV的构建研究

王舒 ^1,2 ,
王华林 ¹ ,
邓菲 ¹ ,
吴东 ¹ ,
龙钢 ¹ ,
袁丽 ¹ ,
胡志红 ^1*,,

1.
1.中国科学院武汉病毒研究所无脊椎动物病毒学开放实验室中国科学院分子病毒学重点实验室湖北武汉 2.北京中国科学院研究生院

通讯作者： 胡志红,

摘要

杆状病毒的出芽病毒具有两类不同的膜融合蛋白, 组I类型的杆状病毒利用的是膜融合蛋白GP64, 而组II类型的杆状病毒利用的是膜融合蛋白F。本文以组II类型的HaSNPV为研究对象, 研究组I类病毒的GP64能否在组II类病毒中正确表达和包装。利用Bac to Bac系统, 构建了带有AcMNPV膜融合蛋白GP64 和增强型绿色荧光蛋白的重组病毒HaSNPVgp64+ egfp+, 同时构建了仅带有增强型绿色荧光蛋白的对照重组病毒HaSNPVegfp+, 通过对HaSNPVgp64+egfp+感染的HzAM1细胞及所产生的子代BV的Western blot检测, 证明GP64可在HzAM1中表达, 并被包装入子代BV。
- 杆状病毒
- , 膜融合蛋白
- , GP64
- , F蛋白

Construction of a Recombinant HaSNPV Expressing GP64

WANG Shu ^1,2 ,
WANG Hua-lin ¹ ,
DENG Fei ¹ ,
WU Dong ¹ ,
LONG Gang ¹ ,
YUAN Li ¹ ,
HU Zhi-hong ^1*,,

Corresponding author: HU Zhi-hong,

Abstract

There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using Bac-to-Bac system, we successfully constructed recombinant baculoviruses HaSNPVgp64~+egfp~+ possessing GP64 of AcMNPV and EGFP, and HaSNPVegfp~+ with an EGFP as control. Western blotting analysis indicated that GP64 was expressed in HzAM1 cells infected with HaSNPVgp64~+egfp~+ and was packaged into progeny BVs.
- Baculovirus
- , Envelope fusion protein （EFP）
- , GP64
- , F protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of a Recombinant HaSNPV Expressing GP64

Corresponding author: HU Zhi-hong,

Abstract: There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using Bac-to-Bac system, we successfully constructed recombinant baculoviruses HaSNPVgp64~+egfp~+ possessing GP64 of AcMNPV and EGFP, and HaSNPVegfp~+ with an EGFP as control. Western blotting analysis indicated that GP64 was expressed in HzAM1 cells infected with HaSNPVgp64~+egfp~+ and was packaged into progeny BVs.