Preparation of SV40 Inactivation Vaccine and Immunization of Balb/c Mice

CAO Zeng; SUN Qiang-ming; SUN Juan; JIANG Hong-chao; LI Hong-zhao; XIE Tian-hong; SUN Mao-sheng; DAI Chang-bai

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April 2005

CAO Zeng, SUN Qiang-ming, SUN Juan, JIANG Hong-chao, LI Hong-zhao, XIE Tian-hong, SUN Mao-sheng and DAI Chang-bai. Preparation of SV40 Inactivation Vaccine and Immunization of Balb/c Mice[J]. Virologica Sinica, 2005, 20(2): 159-163.

Citation: CAO Zeng, SUN Qiang-ming, SUN Juan, JIANG Hong-chao, LI Hong-zhao, XIE Tian-hong, SUN Mao-sheng, DAI Chang-bai. Preparation of SV40 Inactivation Vaccine and Immunization of Balb/c Mice .VIROLOGICA SINICA, 2005, 20(2) : 159-163.

SV40灭活疫苗的制备及其对小鼠免疫的研究

曹增 ¹ ,
孙强明 ¹ ,
孙娟 ² ,
蒋鸿超 ¹ ,
李洪钊 ¹ ,
谢天宏 ¹ ,
孙茂盛 ^1* ,
戴长柏 ¹

1.
1.中国医学科学院中国协和医科大学医学生物学研究所分子生物室云南昆明 2.中国医学科学院中国协和医科大学天津血液学研究所及血液病医院天津

摘要

猿猴空泡病毒40(Simian vacuolating virus 40,SV40) 属于乳多空病毒科,是一种DNA肿瘤病毒。亚洲猿类特别是恒河猴是SV40的天然宿主。感染SV40病毒可导致猴体急性病变或呈长期带毒状态,此外能诱使幼鼠产生肿瘤,并能使多种培养细胞发生转化。本研究初步建立了SV40 病毒在Vero细胞中的增殖培养方法,并且初步建立了β丙内脂灭活病毒的方法和纯化工艺。使用SV40病毒灭活疫苗对Balb/c小鼠进行了免疫,结果表明该疫苗具有较好的免疫原性。随后对SV40 病毒DNA在免疫小鼠的重要脏器中的整合情况进行了调查,结果表明SV40病毒DNA未在小鼠重要脏器中整合。本研究为SV40病毒灭活疫苗的研制和进一步开展猴体抗SV40 感染实验奠定了良好的基础。
- 猴空泡病毒
- , 灭活疫苗
- , β丙内脂
- , Vero细胞

Preparation of SV40 Inactivation Vaccine and Immunization of Balb/c Mice

Abstract

Simian vacuolating virus 40(SV40)belongs to polyoma virus and is a DNA tumor virus.The natural hosts for SV40 are species of Asian macaque monkeys, especially the rhesus (Maccaca mulatta). SV40 can cause widespread infections leading to acutely pathological changes and long-term virus schleping in healthy monkeys. SV40 was shown to be tumorigenic in rodents and was able to transform many types of cells in culture. This work represented the first description of inactivated SV40 by beta-propiolactone and evaluated its immunogenic properties in mice. This work was a primary attempt to tackle SV40 infection and spread has made it ready to further investigate the immunological characteristics of the inactivated virus in monkeys and provided a foundation for vaccine development against SV40.
- SV40
- , Inactivation vaccine
- , Beta-propiolactone
- , Vero cell.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation of SV40 Inactivation Vaccine and Immunization of Balb/c Mice

Abstract: Simian vacuolating virus 40(SV40)belongs to polyoma virus and is a DNA tumor virus.The natural hosts for SV40 are species of Asian macaque monkeys, especially the rhesus (Maccaca mulatta). SV40 can cause widespread infections leading to acutely pathological changes and long-term virus schleping in healthy monkeys. SV40 was shown to be tumorigenic in rodents and was able to transform many types of cells in culture. This work represented the first description of inactivated SV40 by beta-propiolactone and evaluated its immunogenic properties in mice. This work was a primary attempt to tackle SV40 infection and spread has made it ready to further investigate the immunological characteristics of the inactivated virus in monkeys and provided a foundation for vaccine development against SV40.