Establishment of a RT-PCR Assay for the BLSV Gene and the Molecular Detection of BLS

MA Yu-ling; YANG De-ji*; LU Cheng-ping

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April 2005

MA Yu-ling, YANG De-ji* and LU Cheng-ping. Establishment of a RT-PCR Assay for the BLSV Gene and the Molecular Detection of BLS[J]. Virologica Sinica, 2005, 20(2): 197-199.

Citation: MA Yu-ling, YANG De-ji*, LU Cheng-ping. Establishment of a RT-PCR Assay for the BLSV Gene and the Molecular Detection of BLS .VIROLOGICA SINICA, 2005, 20(2) : 197-199.

鸡大肝大脾病毒RT-PCR检测方法的建立及其检测

1.
南京农业大学农业部动物疫病与免疫重点实验室江苏南京

摘要

鸡大肝大脾病(Big liver and spleen disease,BLS)是发生于商品代肉用产蛋母鸡的一种传染性疾病,1980 年最先在澳大利亚发现, 1988 年 Han dlinger[1]等对该病进行了描述。感染鸡直到性成熟后才出现临床症状,仅表现为精神沉郁、产蛋下降或达不到产蛋高峰。死亡率为 1%。...
- 鸡大肝大脾病毒（BLSV）
- , 逆转录多聚酶链式反应（RTPCR）
- , 检测

Establishment of a RT-PCR Assay for the BLSV Gene and the Molecular Detection of BLS

Abstract

Based on the reported Big liver and spleen disease virus (BLSV) gene sequence, a pair of primers was designed and a 375bp product was amplified from the total RNA extracted from homogenates of BLSV-infected livers. Twenty liver samples were randomly collected from two commercial broiler breeder flocks in Nanjing.One of ten liver samples was positive reaction in RT-PCR in one flock but not in another. The RT-PCR detection for cDNA from BLSV was specific with the sensitivity of 4.16×10~(-3)μg/μL. Phylogentic tree analysis suggested that the 338bp sequence of the isolated strain (BLSV-NJ2002) shared 97.3% and 83.6% nt hemologue indentities with that of Australia strain and avian HEV respectively.
- Big liver and spleen disease virus（BLSV）
- , Retrotranscription polymerase chain reaction
- , Detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Establishment of a RT-PCR Assay for the BLSV Gene and the Molecular Detection of BLS

Abstract: Based on the reported Big liver and spleen disease virus (BLSV) gene sequence, a pair of primers was designed and a 375bp product was amplified from the total RNA extracted from homogenates of BLSV-infected livers. Twenty liver samples were randomly collected from two commercial broiler breeder flocks in Nanjing.One of ten liver samples was positive reaction in RT-PCR in one flock but not in another. The RT-PCR detection for cDNA from BLSV was specific with the sensitivity of 4.16×10~(-3)μg/μL. Phylogentic tree analysis suggested that the 338bp sequence of the isolated strain (BLSV-NJ2002) shared 97.3% and 83.6% nt hemologue indentities with that of Australia strain and avian HEV respectively.