ZHANG Ji-yuan, DING Jia-bo, CUI Zhi-zhong, JIANG Shi-jin and Bublot Michel. Comparison of 1.8kb gene family of Marek’s Disease Viruses of Different Pathotypes[J]. Virologica Sinica, 2005, 20(3): 277-282.
Citation: ZHANG Ji-yuan, DING Jia-bo, CUI Zhi-zhong, JIANG Shi-jin, Bublot Michel. Comparison of 1.8kb gene family of Marek’s Disease Viruses of Different Pathotypes .VIROLOGICA SINICA, 2005, 20(3) : 277-282.

不同致病型马立克氏病病毒1.8kb基因家族序列比较

  • 为了分析鸡马立克氏病病毒(Marek′sdiseasevirus,MDV)的致病性与其1.8kb基因家族的关系,本研究比较了四个不同致病型12个毒株的该基因家族的同源性关系,即弱毒疫苗株、强毒株、超强毒株、特超强毒株和中国野毒株。结果表明:不同毒株间1.8kb基因家族的上游调控序列的同源性在1.8kb基因家族转录方向上大于92.5%,序列间有缺失突变,其中大多数突变发生在弱毒株上。CVI988在TATAbox和上游SP1位点间丢失小片段“5′CTCGG3′”。1.8kb基因家族中存在132bp串连重复序列,CVI988包含37个132bp串连重复序列拷贝,强毒株、超强毒株、特超强毒株通常只包含2个串连重复序列。但是已知的中国疫苗毒株814株也只有2个重复序列,表明重复序列的拷贝数不是引起病毒毒力变化的主要原因。各毒株的1.8kb基因家族同源性在97%以上。其中未剪切的1.69kbcDNA中有两个阅读框,分别编码63和64个氨基酸组成的多肽。不同毒株的这二个多肽都很保守,虽然也有一些氨基酸变异,但这些变异与病毒的致病型或地域分布没有明显的关系。

Comparison of 1.8kb gene family of Marek’s Disease Viruses of Different Pathotypes

  • In this study, sequences of the 1.8kb gene family of 12 different strains of Marek′s disease vivuses were compared. These strains covered 4 different pathotypes, i.e, vaccine, virulent, very virulent, and very virulent plus strains, plus three field strains isolated in China. The results demonstrated that the homology of the upstream regulating sequences of the gene family was more than 92.5% among 12 strains, vaccine strain CVI988/Rispens had more mutations especially with a “CTCGG" deletion between TATA box and a SP1 enhancer. There were 3-7 consecutive repeats of 132 bp in the 1.8 kb gene family of vaccine strain CVI988/Rispens, but only 2 copies were detected in the left strains of all pathotypes. However, there were also only 2 copies of 132 bp repeats found even in Chinese vaccine strain 814, indicating that expansion of 132 bp repeat copies did not have an important role as suggested before. The homology of 1.8 kb gene family was more than 97% among different strains. There were 2 ORF in the 1.69 kb no

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    Comparison of 1.8kb gene family of Marek’s Disease Viruses of Different Pathotypes

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    Abstract: In this study, sequences of the 1.8kb gene family of 12 different strains of Marek′s disease vivuses were compared. These strains covered 4 different pathotypes, i.e, vaccine, virulent, very virulent, and very virulent plus strains, plus three field strains isolated in China. The results demonstrated that the homology of the upstream regulating sequences of the gene family was more than 92.5% among 12 strains, vaccine strain CVI988/Rispens had more mutations especially with a “CTCGG" deletion between TATA box and a SP1 enhancer. There were 3-7 consecutive repeats of 132 bp in the 1.8 kb gene family of vaccine strain CVI988/Rispens, but only 2 copies were detected in the left strains of all pathotypes. However, there were also only 2 copies of 132 bp repeats found even in Chinese vaccine strain 814, indicating that expansion of 132 bp repeat copies did not have an important role as suggested before. The homology of 1.8 kb gene family was more than 97% among different strains. There were 2 ORF in the 1.69 kb no

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