Cloning and Sequence Analysis of Component 5 of Banana Bunchy Top Virus NS Strain

ZHENG Yun; RUAN Xiao-lei; LI Hua-ping*; XIAO Huo-gen; FAN Huai-zhong

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June 2005

ZHENG Yun, RUAN Xiao-lei, LI Hua-ping*, XIAO Huo-gen and FAN Huai-zhong. Cloning and Sequence Analysis of Component 5 of Banana Bunchy Top Virus NS Strain[J]. Virologica Sinica, 2005, 20(3): 311-314.

Citation: ZHENG Yun, RUAN Xiao-lei, LI Hua-ping*, XIAO Huo-gen, FAN Huai-zhong. Cloning and Sequence Analysis of Component 5 of Banana Bunchy Top Virus NS Strain .VIROLOGICA SINICA, 2005, 20(3) : 311-314.

香蕉束顶病毒NS株DNA组分5的克隆和序列分析

1.
华南农业大学植物病毒研究室广州

摘要

本文利用PCR技术扩增得到香蕉束顶病毒(BBTV)NS株DNA组分5的全基因,该基因全长为1014nt,具有一个开放阅读框,编码146个氨基酸,蛋白质二级结构包括6个α螺旋,7个β折叠。NS株系与南太平洋组澳大利亚、夏威夷、埃及分离物DNA组分5核苷酸和编码的氨基酸序列相比较,核苷酸序列同源率介于88%～89%之间,氨基酸序列同源率介于80%～88%之间。NS株系与其它分离物在编码成视网膜细胞瘤蛋白(Retinoblastomaprotein,Rb)的基元序列“LXCDE”附近的二级结构上表现明显的差异,推测这种差异可能影响与植物中Rb蛋白的结合效率。
- 香蕉束顶病毒
- , DNA组分5
- , 序列分析

Cloning and Sequence Analysis of Component 5 of Banana Bunchy Top Virus NS Strain

Abstract

The DNA component 5 (DNA-5) of Banana bunchy top virus NS strain was cloned and sequenced. It was 1014nts in full length and could code for 146 amino acids. The nucleotide and amino acid sequences of the DNA-5 were compared with those of isolates from Australia, Hawaii, Egypt, and identities of nucleotide and amino acid sequence among these isolates were between 88%~89% and 80%~88%respectively. The secondary structure near the “LXCDE” motif was much different between NS strain and others. It was deduced that efficiency of binding Rb protein would be influenced by this kind of difference.
- Banana bunchy top virus
- , DNA-5 component
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequence Analysis of Component 5 of Banana Bunchy Top Virus NS Strain

Abstract: The DNA component 5 (DNA-5) of Banana bunchy top virus NS strain was cloned and sequenced. It was 1014nts in full length and could code for 146 amino acids. The nucleotide and amino acid sequences of the DNA-5 were compared with those of isolates from Australia, Hawaii, Egypt, and identities of nucleotide and amino acid sequence among these isolates were between 88%~89% and 80%~88%respectively. The secondary structure near the “LXCDE” motif was much different between NS strain and others. It was deduced that efficiency of binding Rb protein would be influenced by this kind of difference.