Inhibition of Influenza A Virus Replication by Short Hairpin RNA Expression Plasmid

HUANG Juan; JIANG Ping*; GU Xiao-xue; XU Jia-rong; LI Yu-feng

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June 2005

HUANG Juan, JIANG Ping*, GU Xiao-xue, XU Jia-rong and LI Yu-feng. Inhibition of Influenza A Virus Replication by Short Hairpin RNA Expression Plasmid[J]. Virologica Sinica, 2005, 20(3): 326-328.

Citation: HUANG Juan, JIANG Ping*, GU Xiao-xue, XU Jia-rong, LI Yu-feng. Inhibition of Influenza A Virus Replication by Short Hairpin RNA Expression Plasmid .VIROLOGICA SINICA, 2005, 20(3) : 326-328.

质粒介导的核衣壳蛋白siRNA干扰A型流感病毒复制的研究

1.
南京农业大学农业部动物疫病诊断与免疫重点开放实验室江苏南京

摘要

A型流感病毒(Influenzavirus)属于正粘病毒科流感病毒属,可引起鸟类、多种禽类、人类和低等哺乳动物的严重疾病[1],该病毒基因组为负链单股RNA病毒,分8个节段,容易引起抗原漂移和抗原变异,现有的疫苗和药物不能有效的控制该病毒感染。RNA干扰是由双链RNA(dsRNA)引发的信使RNA(mR...
- A型流感病毒
- , 核衣壳蛋白基因
- , RNA干扰

Inhibition of Influenza A Virus Replication by Short Hairpin RNA Expression Plasmid

Abstract

Influenza A virus is a widespread pathogen,but current vaccines and drug therapy are of limited value. RNAi may provid a novel route to control it since RNAi can inhibit gene expression high-efficiently and specificly. A recombinant plasmid with a short hairpin RNA(shRNA)targeting at the nuclear protein gene of the virus was constructed and identified by enzyme digestion and sequence analysis.The hemagglutination(HA) titre and TCID 50 of the virus in chicken embryo fibroblastic(CEF)cells transfected with the plasmid was lower than that nontransfected with the plasmid.It showed that the plasmid could specifictly inhibit the virus production in CEF cells.
- Influenza virus
- , Nuclear protein gene
- , RNA interfering

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Inhibition of Influenza A Virus Replication by Short Hairpin RNA Expression Plasmid

Abstract: Influenza A virus is a widespread pathogen,but current vaccines and drug therapy are of limited value. RNAi may provid a novel route to control it since RNAi can inhibit gene expression high-efficiently and specificly. A recombinant plasmid with a short hairpin RNA(shRNA)targeting at the nuclear protein gene of the virus was constructed and identified by enzyme digestion and sequence analysis.The hemagglutination(HA) titre and TCID 50 of the virus in chicken embryo fibroblastic(CEF)cells transfected with the plasmid was lower than that nontransfected with the plasmid.It showed that the plasmid could specifictly inhibit the virus production in CEF cells.