LIU Juan, SUN Yong-tao, WANG Shao-yang, SHI Meng-yuan, ZHUANG Yan and ZHAI Song. Studies on the Inhibitory Effects of HNP1,3 on HSV1 Infection in vitro[J]. Virologica Sinica, 2005, 20(4): 366-369.
Citation: LIU Juan, SUN Yong-tao, WANG Shao-yang, SHI Meng-yuan, ZHUANG Yan, ZHAI Song. Studies on the Inhibitory Effects of HNP1,3 on HSV1 Infection in vitro .VIROLOGICA SINICA, 2005, 20(4) : 366-369.

人中性粒细胞多肽HNP_(1,3)体外抗单纯疱疹病毒Ⅰ型的作用

  • 体外观察人中性粒细胞多肽1,3(Humanneutrophilpeptide,HNP1,3)及阿昔洛韦(Acyclovir,ACV)对单纯疱疹病毒-Ⅰ型(Herpessimplexvirus1,HSV-1)的抑制作用。以Vero细胞为靶细胞,用各种浓度HNP1,3与游离病毒颗粒(直接失活组)及感染病毒后的靶细胞(复制抑制组)进行相互作用,镜下观察各药物对HSV-1致细胞病变效应的抑制作用,并采用ELISA法测定感染48h后药物对HSV-1囊膜糖蛋白分泌的抑制作用。MTT法检测各药物对细胞的毒性作用。结果显示直接失活组中,HNP1,3可使HSV-1的致细胞病变效应减轻,对HSV-1直接失活的50%有效浓度(EC50)为8.1μg/mL、10.03μg/mL;复制抑制组中,ACV使HSV-1的致细胞病变效应减轻,EC50为0.68μg/mL。MTT检测结果表明HNP1,3在治疗浓度范围内无明显细胞毒性。以上结果表明HNP1,3除具有较强的抗菌作用和抗人类免疫缺陷病毒Ⅰ型(Humanimmunodeficiencyvirus1,HIV-1)活性外,还能失活HSV-1病毒颗粒,从而逆转病毒及其蛋白的病毒效应(致细胞病变)和抑制病毒蛋白质的合成。

Studies on the Inhibitory Effects of HNP1,3 on HSV1 Infection in vitro

  • In order to evaluate the effect of human neutrophil peptide_{1,3}(HNP_{1,3})and acyclovir (ACV) on Herpes simplex virus type 1 (HSV-1) infection, Vero cells were inoculated with HSV-1. Antiviral activity was evaluated by reduction in cytopathic effect and HSV-1 envelop glycoprotein antigen,which determined by sandwich ELISA 48h after infection when drug~3 were interacted with free virus particles and infected vero cells(group A: inactivation of viruses, group B: inhibition of viral replication). Their cytoxicity were also measured by MTT assay. The results showed HNP_{1,3} reversed HSV-1 induced cytopathic effect and inhibited the antigen expression in group A, their 50% efficiency concentrations ( EC_{50}) were 8.1μg/mL、10.03μg/mL;In group B,ACV can lessen HSV-1 induced cytopathicity effects and reduced the antigen secretion whose EC_{50} was 0.68μg/mL. No cytotoxic effects of HNP_\{1,3\} were found by MTT assay. These findings confirmed that HNP_{1,3}, previously showed active to bacterium and human immunod

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    Studies on the Inhibitory Effects of HNP1,3 on HSV1 Infection in vitro

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    Abstract: In order to evaluate the effect of human neutrophil peptide_{1,3}(HNP_{1,3})and acyclovir (ACV) on Herpes simplex virus type 1 (HSV-1) infection, Vero cells were inoculated with HSV-1. Antiviral activity was evaluated by reduction in cytopathic effect and HSV-1 envelop glycoprotein antigen,which determined by sandwich ELISA 48h after infection when drug~3 were interacted with free virus particles and infected vero cells(group A: inactivation of viruses, group B: inhibition of viral replication). Their cytoxicity were also measured by MTT assay. The results showed HNP_{1,3} reversed HSV-1 induced cytopathic effect and inhibited the antigen expression in group A, their 50% efficiency concentrations ( EC_{50}) were 8.1μg/mL、10.03μg/mL;In group B,ACV can lessen HSV-1 induced cytopathicity effects and reduced the antigen secretion whose EC_{50} was 0.68μg/mL. No cytotoxic effects of HNP_\{1,3\} were found by MTT assay. These findings confirmed that HNP_{1,3}, previously showed active to bacterium and human immunod

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