Co-expression and Construction of Eukaryote Plasmid of pp38 and pp24 of Marek’s Disease . Virus

JIANG Shi-jin; DING Jia-bo; MENG Shan-shan; CUI Zhi-zhong; YANG Han-chun

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August 2005

JIANG Shi-jin, DING Jia-bo, MENG Shan-shan, CUI Zhi-zhong and YANG Han-chun. Co-expression and Construction of Eukaryote Plasmid of pp38 and pp24 of Marek’s Disease . Virus[J]. Virologica Sinica, 2005, 20(4): 404-407.

Citation: JIANG Shi-jin, DING Jia-bo, MENG Shan-shan, CUI Zhi-zhong, YANG Han-chun. Co-expression and Construction of Eukaryote Plasmid of pp38 and pp24 of Marek’s Disease . Virus .VIROLOGICA SINICA, 2005, 20(4) : 404-407.

Ⅰ型马立克氏病病毒pp38和pp24基因的真核共表达

姜世金 ¹ ,
丁家波 ¹ ,
孟珊珊 ¹ ,
崔治中 ^1* ,
杨汉春 ²

1.
1.山东农业大学动物科技学院山东泰安 2.中国农业大学动物医学院北京

摘要

本研究将Ⅰ型超强毒MDVMd11株的pp38和pp24完整基因分别克隆到真核双表达载体pBudCE4.1中,在脂质体作用下将阳性克隆DNA转染CEF,通过间接免疫荧光试验(IFA)用单克隆抗体H19和鼠抗GST-pp24血清分别检测到了pp38和pp24基因的单独表达。然后将MDVMd11株的pp38和pp24完整基因同时克隆到载体pBudCE4.1中,在脂质体作用下将阳性克隆DNA转染CEF,通过IFA检测和用抗pp24多克隆血清进行West-ern-blotting试验检测到了PP38和PP24磷蛋白的共表达。
- 马立克氏病病毒
- , PP38
- , PP24
- , 共表达

Co-expression and Construction of Eukaryote Plasmid of pp38 and pp24 of Marek’s Disease . Virus

Abstract

pp38 gene and pp24 gene of MDV Md11 strain were cloned into pBudCE(4.1) vector respectively. The pBudCE(4.1)-pp38 and pBudCE(4.1)-pp24 eukaryotic expressing plasmids were transfected into CEF respectively by lipofectamine reagent. 24, 48, 72 hours after transfection, the expression of pp38 was testified with Mab H19, and pp24 with the antiserum of pp24 expressed in E.coli.pp38 gene and pp24 gene were cloned into pBudCE(4.1) vector which can express two different genes at the same time. After transfection,the co-expression of PP38 and PP24 was testified by IFA and by Western-blotting with Anti-GST-pp24 sera.
- Marek′s disease virus （MDV）
- , Phosphoprotein 38 （PP38）
- , Phosphoprotein 24 （PP24）
- , Co-expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Co-expression and Construction of Eukaryote Plasmid of pp38 and pp24 of Marek’s Disease . Virus

Abstract: pp38 gene and pp24 gene of MDV Md11 strain were cloned into pBudCE(4.1) vector respectively. The pBudCE(4.1)-pp38 and pBudCE(4.1)-pp24 eukaryotic expressing plasmids were transfected into CEF respectively by lipofectamine reagent. 24, 48, 72 hours after transfection, the expression of pp38 was testified with Mab H19, and pp24 with the antiserum of pp24 expressed in E.coli.pp38 gene and pp24 gene were cloned into pBudCE(4.1) vector which can express two different genes at the same time. After transfection,the co-expression of PP38 and PP24 was testified by IFA and by Western-blotting with Anti-GST-pp24 sera.