Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques

WANG Jin-zhong; JIA Hui; WEN Si-yuan; WANG Shen-qi; ZHANG Min-zhao; ZHAO Xiang-yun; DONG jin-gao

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August 2005

WANG Jin-zhong, JIA Hui, WEN Si-yuan, WANG Shen-qi, ZHANG Min-zhao, ZHAO Xiang-yun and DONG jin-gao. Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques[J]. Virologica Sinica, 2005, 20(4): 429-433.

Citation: WANG Jin-zhong, JIA Hui, WEN Si-yuan, WANG Shen-qi, ZHANG Min-zhao, ZHAO Xiang-yun, DONG jin-gao. Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques .VIROLOGICA SINICA, 2005, 20(4) : 429-433.

百合病毒的DNA芯片检测技术研究

王进忠 ^1,2* ,
贾慧 ¹ ,
文思远 ³ ,
王升启 ³ ,
张民照 ¹ ,
赵祥云 ² ,
董金皋 ⁴

1.
1.北京北京农学院植物科学技术系 2.北京市农业应用新技术重点实验室 3.军事医学科学院放射医学研究所北京 4.河北农业大学生命科学学院河北保定

摘要

根据已知的黄瓜花叶病毒,百合无症病毒、百合斑驳病毒基因核苷酸序列,设计引物和探针,制备寡核苷酸芯片。用Cy3标记核苷酸引物,不对称RT-PCR扩增产物与芯片上的寡核苷酸探针杂交,荧光扫描仪检测并分析信号。研究制备的基因芯片能够检测侵染百合的3种重要病毒核酸的特异性荧光信号,该项技术具有特异、灵敏、快速的优点。
- 黄瓜花叶病毒
- , 百合无症病毒
- , 百合斑驳病毒
- , 基因芯片
- , 检测

Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques

Abstract

Specific primers and probes were designed according to conservative and specific DNA sequence of CMV,LSV and LMoV.The probes were spotted on glass slides to form microarrays The Cy3-labled single strand DNA fragments prepared by dissymmetical RT-PCR were hybridized with the probes on the glass slides .The microarrays were scanned and analyzed with a scanner. DNA microarray could detect different typed DNA of CMV,LSV and LMoV with adequate specificity and sensitivity . Microarray techniques establish a rapid,sensitive and specific diagnostic assay for lily viruses. The successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention ,and widely applied in specific pathogen detection of diseases such as lily virus.
- Cucumber mosaic virus（CMV）
- , Lily symptomless virus（LSV）
- , Lily mottle virus（LMoV）
- , DNA microarry Detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection for CMV,LSV,LMoV Infected Lily with DNA Microarry Techniques

Abstract: Specific primers and probes were designed according to conservative and specific DNA sequence of CMV,LSV and LMoV.The probes were spotted on glass slides to form microarrays The Cy3-labled single strand DNA fragments prepared by dissymmetical RT-PCR were hybridized with the probes on the glass slides .The microarrays were scanned and analyzed with a scanner. DNA microarray could detect different typed DNA of CMV,LSV and LMoV with adequate specificity and sensitivity . Microarray techniques establish a rapid,sensitive and specific diagnostic assay for lily viruses. The successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention ,and widely applied in specific pathogen detection of diseases such as lily virus.