Isolation and Identification of Porcine Circovirus Type 2 from Sichuan Province and Sequence Analysis of ORF2 Gene

WANG Xin; GUO Wan-zhu*; ZHU Ling; XU Zhi-wen; YAN Qi-gui; WANG Yin; WANG Xiao-yu; LUO Yan; ZHOU Ting

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August 2005

WANG Xin, GUO Wan-zhu*, ZHU Ling, XU Zhi-wen, YAN Qi-gui, WANG Yin, WANG Xiao-yu, LUO Yan and ZHOU Ting. Isolation and Identification of Porcine Circovirus Type 2 from Sichuan Province and Sequence Analysis of ORF2 Gene [J]. Virologica Sinica, 2005, 20(4): 438-440.

Citation: WANG Xin, GUO Wan-zhu*, ZHU Ling, XU Zhi-wen, YAN Qi-gui, WANG Yin, WANG Xiao-yu, LUO Yan, ZHOU Ting. Isolation and Identification of Porcine Circovirus Type 2 from Sichuan Province and Sequence Analysis of ORF2 Gene .VIROLOGICA SINICA, 2005, 20(4) : 438-440.

猪II型圆环病毒四川分离株鉴定及其ORF2基因序列分析

1.
四川农业大学动物生物技术中心四川雅安

摘要
- 猪II型圆环病毒（PCV-2）
- , 分离
- , ORF2基因
- , 序列分析

Isolation and Identification of Porcine Circovirus Type 2 from Sichuan Province and Sequence Analysis of ORF2 Gene

Abstract

The PCV-2 positive tissues by PCR-restriction fragment length polymorphism (PCR-RFLP) were inoculated to the PK-15 cells not contaminated by PCV. The viral particles were observed in the infected PK-15 cells by eletronmicroscope .It was concluded that the isolate was PCV-2 and was named SCH-A strain.The capsid protein gene(cp) in ORF2 was amplified from PK-15 cell line infected with SCH-A strain by PCR, and was cloned and sequenced.The nucleotide sequences of ORF2 gene of the SCH-A strain was compared with other porcine circovirus isolates.The results showed that the homologies of nucleotides were between 90.5% and 96.7% in comparision with the PCV-2 reference strains,but the homologies of nucleotides were only about 67% with the PCV-1 reference strains. SCH-A strain displayed the highest nucleotide homology((96.7)%) with the reference strain Hunan .Phylogenetic tree analysis showed ORF2 gene of the SCH-A isolate belongs to PCV-2 .
- Porcine circovirus type 2（PCV-2）
- , Isolation ORF2 gene
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Identification of Porcine Circovirus Type 2 from Sichuan Province and Sequence Analysis of ORF2 Gene

Abstract: The PCV-2 positive tissues by PCR-restriction fragment length polymorphism (PCR-RFLP) were inoculated to the PK-15 cells not contaminated by PCV. The viral particles were observed in the infected PK-15 cells by eletronmicroscope .It was concluded that the isolate was PCV-2 and was named SCH-A strain.The capsid protein gene(cp) in ORF2 was amplified from PK-15 cell line infected with SCH-A strain by PCR, and was cloned and sequenced.The nucleotide sequences of ORF2 gene of the SCH-A strain was compared with other porcine circovirus isolates.The results showed that the homologies of nucleotides were between 90.5% and 96.7% in comparision with the PCV-2 reference strains,but the homologies of nucleotides were only about 67% with the PCV-1 reference strains. SCH-A strain displayed the highest nucleotide homology((96.7)%) with the reference strain Hunan .Phylogenetic tree analysis showed ORF2 gene of the SCH-A isolate belongs to PCV-2 .