CHEN Yong-jun, SU Xin-ming, GAO Xiao-fei, ZHENG Sheng-qi, YU Chun-mei, CAO Rui-bing, ZHOU Bin and CHEN Pu-yan*. Preliminary Studies of Prokaryotic Expression of GP5 of Porcine Reproductive and Respiratory Syndrome Virus[J]. Virologica Sinica, 2005, 20(4): 441-443.
Citation: CHEN Yong-jun, SU Xin-ming, GAO Xiao-fei, ZHENG Sheng-qi, YU Chun-mei, CAO Rui-bing, ZHOU Bin, CHEN Pu-yan*. Preliminary Studies of Prokaryotic Expression of GP5 of Porcine Reproductive and Respiratory Syndrome Virus .VIROLOGICA SINICA, 2005, 20(4) : 441-443.

猪繁殖与呼吸综合症病毒GP5蛋白原核表达初步研究

Preliminary Studies of Prokaryotic Expression of GP5 of Porcine Reproductive and Respiratory Syndrome Virus

  • The envelope glycoprotein (GP5) is one of the major structural proteins of Porcine reproductive and respiratory syndrome virus (PRRSV). In this report, the N-terminal hydrophobic sequence of envelope glycoprotein gene (orf5) was deleted by PCR. The gene was subcloned into prokaryotic expressing vector pET-28 a(+) , the recombinant plasmid named pET-GP5 was transformed into E.coli cell BL21. SDS-PAGE and Western-blot indicated that the orf5 gene was expressed successfully and the recombinant fusion protein, which was about 20.8kDa, had immunologically reactive activity. The studies lay foundations for further study on the development of vaccines for PRRSV.

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    Preliminary Studies of Prokaryotic Expression of GP5 of Porcine Reproductive and Respiratory Syndrome Virus

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    Abstract: The envelope glycoprotein (GP5) is one of the major structural proteins of Porcine reproductive and respiratory syndrome virus (PRRSV). In this report, the N-terminal hydrophobic sequence of envelope glycoprotein gene (orf5) was deleted by PCR. The gene was subcloned into prokaryotic expressing vector pET-28 a(+) , the recombinant plasmid named pET-GP5 was transformed into E.coli cell BL21. SDS-PAGE and Western-blot indicated that the orf5 gene was expressed successfully and the recombinant fusion protein, which was about 20.8kDa, had immunologically reactive activity. The studies lay foundations for further study on the development of vaccines for PRRSV.

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