Expression，Purification and Antigenicity Analysis of the HDV Antigen

XIE Li; HUANG De-zhuang; HE Li-xiang; ZHOU Yu-sen

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August 2005

XIE Li, HUANG De-zhuang, HE Li-xiang, ZHOU Yu-sen and Expression，Purification and Antigenicity Analysis of the HDV Antigen[J]. Virologica Sinica, 2005, 20(4): 444-446.

Citation: XIE Li, HUANG De-zhuang, HE Li-xiang, ZHOU Yu-sen, . Expression，Purification and Antigenicity Analysis of the HDV Antigen .VIROLOGICA SINICA, 2005, 20(4) : 444-446.

丁型肝炎病毒抗原的表达纯化和抗原性分析

谢立 ^1,3 ,
黄德庄 ¹ ,
贺立香 ¹ ,
罗朝霞 ¹ ,
周育森 ²

1.
1.北京北京市卫生局肝炎研究所 2.军事医学科学院微生物流行病研究所 3.中国科学院研究生院

摘要
- 丁型肝炎病毒
- , 基因克隆和表达
- , 丁肝抗原

Expression，Purification and Antigenicity Analysis of the HDV Antigen

XIE Li ^1,3 ,
HUANG De-zhuang ¹ ,
HE Li-xiang ¹ ,
ZHOU Yu-sen ¹ ,
²

Abstract

Amplified HDV fragment by RT-PCR was cloned into T vector and PQE_{31} plasmid, which established a prokaryotic expression vector in E coli M_{15} The recombinant protein was purified by Ni-NTA column affinity chromatography, and identified by Western blot and ELISA: HDV antigen was expressed efficiently and had a well antigenicity character. It can be used in HDV clinical diagnosis and epidemiology survey.
- patitis D virus （HDV）
- , Gene cloning and expressing
- , HDV antigen

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression，Purification and Antigenicity Analysis of the HDV Antigen

Abstract: Amplified HDV fragment by RT-PCR was cloned into T vector and PQE_{31} plasmid, which established a prokaryotic expression vector in E coli M_{15} The recombinant protein was purified by Ni-NTA column affinity chromatography, and identified by Western blot and ELISA: HDV antigen was expressed efficiently and had a well antigenicity character. It can be used in HDV clinical diagnosis and epidemiology survey.