DENG Chun-qing, DENG Guo-hong and WANG Yu-ming*. eNOS Gene (894G/T) Polymorphisms Among Patients Infected With HBV[J]. Virologica Sinica, 2005, 20(5): 476-479.
Citation: DENG Chun-qing, DENG Guo-hong, WANG Yu-ming*. eNOS Gene (894G/T) Polymorphisms Among Patients Infected With HBV .VIROLOGICA SINICA, 2005, 20(5) : 476-479.

e NOS基因(894G/T)多态性与HBV感染

  • 为探讨HBV感染临床转归与eNOS基因多态性的关系,选择传染科门诊及住院部2002.2-2004.2诊治的无亲缘关系的重庆地区汉族居民2400人,采用聚合酶链反应(PCR)及限制性内切酶技术检测eNOS exon7位点894G/T多态性,应用SPSS软件χ2检验进行统计学处理:首先Hardy-Weinberg平衡检验Pa0.01,表明拟合度优良,收集病例有良好的代表性;其次乙肝病毒携带与其余任何组比较(急乙肝与肝癌组因例数太少除外)等位基因频率与基因型频率Pb0.05,乙肝病毒携带+慢乙肝组与肝硬化+重症肝炎组等位基因频率与基因型频率比较Pb0.05。以非条件logistic回归校正年龄及性别因素进行分层分析,在隐性模式和共显性模式下,eNOS基因位点894G/T SNP与HBV感染后疾病的携带状态显著关联。与894G/T和T/T基因型相比,G/G基因型的个体发展为携带状态的易感性显著增高(隐性模式:OR=1.428 95%CI=1.050-1.942;共显性模式:OR=1.310 95%CI=1.025-1.676)。

eNOS Gene (894G/T) Polymorphisms Among Patients Infected With HBV

  • To characterize the relationship between exon7 of eNOS gene(position 894G/T) polymorphism and clinical subgroups infected with HBV.The genotype was analyzed by using method of polymerase chain reaction(PCR),enzyme restriction after PCR amplification,and agrose gel electrophresis.Results show that statistic differences of genotype and allele frequency exist in the patients between AsC(asymptomatic carrier)and any other subgroups;between AsC+CHB(Chronic hepatitis B)and SHB(Severe hepatitis B)+LC(Liver cirrosis) subgroups.Logistic regression analysis supports that G/G genotype of exon7 is more succeptiable to develop Asc state than G/T or T/T genotype.So exon7 of eNOS gene(position 894G/T) polymophism exists among the people infected with HBV,espescially G/G genotype of exon7 is might more succeptiable to develop Asc state than G/T or T/T genotype.

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    eNOS Gene (894G/T) Polymorphisms Among Patients Infected With HBV

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    Abstract: To characterize the relationship between exon7 of eNOS gene(position 894G/T) polymorphism and clinical subgroups infected with HBV.The genotype was analyzed by using method of polymerase chain reaction(PCR),enzyme restriction after PCR amplification,and agrose gel electrophresis.Results show that statistic differences of genotype and allele frequency exist in the patients between AsC(asymptomatic carrier)and any other subgroups;between AsC+CHB(Chronic hepatitis B)and SHB(Severe hepatitis B)+LC(Liver cirrosis) subgroups.Logistic regression analysis supports that G/G genotype of exon7 is more succeptiable to develop Asc state than G/T or T/T genotype.So exon7 of eNOS gene(position 894G/T) polymophism exists among the people infected with HBV,espescially G/G genotype of exon7 is might more succeptiable to develop Asc state than G/T or T/T genotype.

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