Cloning of Porcine Endogenous Retrovirus 5￠-Untranslated Region and Analysis of Its Structure

WU Jian-min; YANG Yu-biao; LV Mao-min; GUO Yan-ru; ZHANG Jin-gang

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October 2005

WU Jian-min, YANG Yu-biao, LV Mao-min, GUO Yan-ru, ZHANG Jin-gang and Cloning of Porcine Endogenous Retrovirus 5￠-Untranslated Region and Analysis of Its Structure[J]. Virologica Sinica, 2005, 20(5): 522-525.

Citation: WU Jian-min, YANG Yu-biao, LV Mao-min, GUO Yan-ru, ZHANG Jin-gang, . Cloning of Porcine Endogenous Retrovirus 5￠-Untranslated Region and Analysis of Its Structure .VIROLOGICA SINICA, 2005, 20(5) : 522-525.

猪内源性反转录病毒5￠端非编码区的克隆及结构分析

吴健敏 ^1,2 ,
阳玉彪 ¹ ,
吕茂民 ¹ ,
谢放 ¹ ,
郭艳茹 ¹ ,
章金刚 ^1*

1.
1.军事医学科学院输血医学研究所北京 2.广西兽医研究所广西南宁

摘要

通过五指山猪内源性反转录病毒5′端非编码区(5′UTR)cDNA的克隆,分析其一级结构和调控元件,为进一步研究其在PERV复制、转录中的调控机制奠定基础。本研究采用cDNA末端快速扩增技术(RACE)获得全长约1035bp的PERV 5′UTR。通过NCBI公共数据库BLASTn序列进行同源性分析,并应用KEGG数据库对该转录调控区进行顺式作用元件定位分析,结果发现PERV 5′UTR与GenBank公布的部分PERV 5′UTR相比较,同源性在82.6~94.8%之间。一级结构分析发现PERV 5′UTR由U3、R、U5区、引物结合区(PBS)及前导序列组成,可能的核心启动子序列与具有增强子作用的39bp重复序列分别位于U3区的-67~+1与-97~-59区段。在5′UTR转录调控区(-428~+507)鉴定出31个有效的顺式作用元件位点,其中NF-Y、TBP、Oct-1、HSF、GATA-1和GATA-2等与PERV的转录、调控密切相关。
- PERV猪内源性反转录病毒
- , 五指山猪
- , 5′端非编码区
- , 一级结构
- , 顺式作用元件

Cloning of Porcine Endogenous Retrovirus 5￠-Untranslated Region and Analysis of Its Structure

WU Jian-min ^1,2 ,
YANG Yu-biao ¹ ,
LV Mao-min ¹ ,
GUO Yan-ru ¹ ,
ZHANG Jin-gang ¹ ,
^1*

Abstract

The full-length 5′-untranslated region(5′UTR) of Porcine endogenous retrovirus(PERV) from Chinese Wuzhi shan(WZS) miniature pigs was cloned by RACE.Seguencing of PERV-WZS 5′UTR showed that it was 1035bp.Compared with other PERV 5’UTR sequences published in GenBank,the homology was 82.6~94.8%.Primary structure analysis revealed that the PERV-WZS 5′UTR was composed of U3,R,U5,primer binding site(PBS) and leader sequence.The potential promoter and enhancer of the 39-bp repeat sequences were located in U3 region(-67~+1) and(-97~-59) respectively.There were 31 potential cis-acting elements such as NF-Y,TBP,Oct-1,HSF,GATA-1,and GATA-2,which were considered to be related to PERV transcription and regulation.
- Porcine endogenus retrovirus（PERV）
- , WZS miniature pigs
- , 5′UTR
- , Primary structure
- , cis-acting elements

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning of Porcine Endogenous Retrovirus 5￠-Untranslated Region and Analysis of Its Structure

Abstract: The full-length 5′-untranslated region(5′UTR) of Porcine endogenous retrovirus(PERV) from Chinese Wuzhi shan(WZS) miniature pigs was cloned by RACE.Seguencing of PERV-WZS 5′UTR showed that it was 1035bp.Compared with other PERV 5’UTR sequences published in GenBank,the homology was 82.6~94.8%.Primary structure analysis revealed that the PERV-WZS 5′UTR was composed of U3,R,U5,primer binding site(PBS) and leader sequence.The potential promoter and enhancer of the 39-bp repeat sequences were located in U3 region(-67~+1) and(-97~-59) respectively.There were 31 potential cis-acting elements such as NF-Y,TBP,Oct-1,HSF,GATA-1,and GATA-2,which were considered to be related to PERV transcription and regulation.