ZHANG Hai-yuan, HUANG Bo-qing, MEI Chun-lei and ZHANG Zhong-xin. Cloning,Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus Cloning,Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus[J]. Virologica Sinica, 2005, 20(5): 534-538.
Citation: ZHANG Hai-yuan, HUANG Bo-qing, MEI Chun-lei, ZHANG Zhong-xin. Cloning,Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus Cloning,Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus .VIROLOGICA SINICA, 2005, 20(5) : 534-538.

SeMNPV组织蛋白酶基因的克隆表达及其对病毒杀虫的影响

  • 对甜菜夜蛾核型多角体病毒(SeMNPV)的组织蛋白酶(v-cath)基因进行了克隆,序列分析及原核表达。此基因编码区长1014bp,预计编码一个338个氨基酸的蛋白产物,其蛋白的大小约42kD。序列分析表明,SeMNPV的V-CATH与其它杆状病毒的同源蛋白具有相似的保守结构,保留有相同的酶活性位点。根据几种已知的杆状病毒组织蛋白酶序列构建进化树,发现SeMNPV的V-CATH位于NPV组中一个单独的分支,它可能拥有特殊的进化历程。使用1.2μL的表达组织蛋白酶菌液和SeNPV共感染3龄末甜菜夜蛾幼虫,幼虫病毒致死率提高9.21%,病毒致死时间提前12 h,原核表达的组织蛋白酶对病毒杀虫速度有一定的影响。

Cloning,Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus Cloning,Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus

  • A putative v-cathepsin(v-cath) gene of Spodoptera exigua nucleopolyhedrovirus(SeMNPV) was cloned,sequenced and expressed.The results showed that the gene size was 1014 nucleotides,encoding a 338 amino acids putative protein.The amino acid sequence alignment of ten baculoviral v-CATHs showed that the primary structure and the catalytic sites were conserved.A pholygenetic tree of these v-CATHs constructed by using maximum parsimony analysis indicated that the v-cath gene of SeMNPV had a different evolutionary history from that of other NPVs.When late 3th instar Spodoptera exigua larvae were infected with SeMNPV combinded with 1.2 μL expressed bacteria and medium,the mortality was increased 9.21%,and LT50 was 12 h shorter than the control.The v-CATH expressed in prokaryote has a significant effect on the killing speed of SeMNPV insecticide.

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    Cloning,Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus Cloning,Expression and Function Analysis of Cathepsin Gene Encoded by Spodoptera exigua Nucleopolyhedrovirus

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    Abstract: A putative v-cathepsin(v-cath) gene of Spodoptera exigua nucleopolyhedrovirus(SeMNPV) was cloned,sequenced and expressed.The results showed that the gene size was 1014 nucleotides,encoding a 338 amino acids putative protein.The amino acid sequence alignment of ten baculoviral v-CATHs showed that the primary structure and the catalytic sites were conserved.A pholygenetic tree of these v-CATHs constructed by using maximum parsimony analysis indicated that the v-cath gene of SeMNPV had a different evolutionary history from that of other NPVs.When late 3th instar Spodoptera exigua larvae were infected with SeMNPV combinded with 1.2 μL expressed bacteria and medium,the mortality was increased 9.21%,and LT50 was 12 h shorter than the control.The v-CATH expressed in prokaryote has a significant effect on the killing speed of SeMNPV insecticide.

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