Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan

CHE Hai-yan; LUO Da-quan*; YANG Xu-guang; FU Rui-yi; YE Sha-bing

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October 2005

CHE Hai-yan, LUO Da-quan*, YANG Xu-guang, FU Rui-yi and YE Sha-bing. Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan[J]. Virologica Sinica, 2005, 20(5): 543-545.

Citation: CHE Hai-yan, LUO Da-quan*, YANG Xu-guang, FU Rui-yi, YE Sha-bing. Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan .VIROLOGICA SINICA, 2005, 20(5) : 543-545.

海南岛番木瓜和扶桑上粉虱传双生病毒的检测及序列分析

1.
中国热带农业科学院环境与植物保护研究所海南儋州

摘要
- 粉虱传双生病毒
- , 海南
- , PCR
- , 序列分析

Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan

Abstract

Three virus-infected Carica papaya samples showing mosaic,leaf roll symptoms and three virus-infected Hibiscus rosa-sinensis samples showing chlorosis and distortion symptoms were collected in Danzhou of Hainan Province.A pair of degenerate primers was designed according to the conserved sequence of CP gene and intergenic spacer of Whitefly-transmittal geminiviruses(WTGV),PCR product of expected size was amplified from six samples.The specific fragments were cloned and sequenced,and sequence analysis showed that PCR product had a geminiviral gene origin,which was demonstrated by BLAST.Partial DNA-A between P1 and H2 shared only 66% nucleotide sequence identity.Polygenie analysis with DNA-A of P1 and H2 from other 16 WTG,P1 was most closely related to LAYVV,H2 differed from those of other geminiviruses and constitute a new evolutionary branch.
- Whitefly-transmitted geminiviruses
- , PCR
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan

Abstract: Three virus-infected Carica papaya samples showing mosaic,leaf roll symptoms and three virus-infected Hibiscus rosa-sinensis samples showing chlorosis and distortion symptoms were collected in Danzhou of Hainan Province.A pair of degenerate primers was designed according to the conserved sequence of CP gene and intergenic spacer of Whitefly-transmittal geminiviruses(WTGV),PCR product of expected size was amplified from six samples.The specific fragments were cloned and sequenced,and sequence analysis showed that PCR product had a geminiviral gene origin,which was demonstrated by BLAST.Partial DNA-A between P1 and H2 shared only 66% nucleotide sequence identity.Polygenie analysis with DNA-A of P1 and H2 from other 16 WTG,P1 was most closely related to LAYVV,H2 differed from those of other geminiviruses and constitute a new evolutionary branch.