RT-PCR Detection of Taura Syndrome Virus in Penaeus vannami from Southeast Seaside of China Virus

HUANG Xin-xin; MO Sheng-lan; LU Cheng-ping*

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October 2005

HUANG Xin-xin, MO Sheng-lan and LU Cheng-ping*. RT-PCR Detection of Taura Syndrome Virus in Penaeus vannami from Southeast Seaside of China Virus[J]. Virologica Sinica, 2005, 20(5): 546-548.

Citation: HUANG Xin-xin, MO Sheng-lan, LU Cheng-ping*. RT-PCR Detection of Taura Syndrome Virus in Penaeus vannami from Southeast Seaside of China Virus .VIROLOGICA SINICA, 2005, 20(5) : 546-548.

RT-PCR法检测我国东南沿海凡纳滨对虾的桃拉综合症病毒

1.
南京农业大学农业部动物疫病诊断与免疫重点实验室江苏南京

摘要
- 凡纳滨对虾
- , RT-PCR
- , 桃拉综合征病毒（TSV）
- , 核酸测序

RT-PCR Detection of Taura Syndrome Virus in Penaeus vannami from Southeast Seaside of China Virus

Abstract

Taura syndrome virus(TSV) is one of the important pathogenic agents of Penaeus vannami,which causes prawn infection with serious disease in China in recent years.245 samples collected from 26 farms of Zhangzhou,Xiamen,Shenzhen,Yangjiang,Ningbo and Guangzhou.were detected for TSV with RT-PCR,the positive rates were 100%,33.3%,40.7%,50% and 40%,respectively.Southern blotting and nucleotide sequencing were performed to confirm the specificity of the amplified RT-PCR products.The homology of 231bp nucleotide sequences reached 98.3%-100% when analysed and compared with the GenBank using the BLAST program.Phylogenetic analyses clustered the TSV isolates into two groups: one contained USA and Chinese Taiwan isolates;the other contained Vietnam isolate(YN1) and 21 isolates of mainland China.The 21 China isolates clustered into one branch,while the YN1 distributed in another branch.5 strains from Guangzhou and 2 strains from Shenzhen constituted one subcluster of the China branch.
- Penaeus vannami
- , RT-PCR
- , Taura syndrome virus（TSV）
- , Nucleotide sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

RT-PCR Detection of Taura Syndrome Virus in Penaeus vannami from Southeast Seaside of China Virus

Abstract: Taura syndrome virus(TSV) is one of the important pathogenic agents of Penaeus vannami,which causes prawn infection with serious disease in China in recent years.245 samples collected from 26 farms of Zhangzhou,Xiamen,Shenzhen,Yangjiang,Ningbo and Guangzhou.were detected for TSV with RT-PCR,the positive rates were 100%,33.3%,40.7%,50% and 40%,respectively.Southern blotting and nucleotide sequencing were performed to confirm the specificity of the amplified RT-PCR products.The homology of 231bp nucleotide sequences reached 98.3%-100% when analysed and compared with the GenBank using the BLAST program.Phylogenetic analyses clustered the TSV isolates into two groups: one contained USA and Chinese Taiwan isolates;the other contained Vietnam isolate(YN1) and 21 isolates of mainland China.The 21 China isolates clustered into one branch,while the YN1 distributed in another branch.5 strains from Guangzhou and 2 strains from Shenzhen constituted one subcluster of the China branch.