Prokaryotic Expression of SARS Coronavirus Nonstructural Protein 9

LIU Jia-sen; CHEN Shu-hong; LIU Huai-ran; PANG Hai; KONG Xian-gang

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October 2005

LIU Jia-sen, CHEN Shu-hong, LIU Huai-ran, PANG Hai, KONG Xian-gang and Prokaryotic Expression of SARS Coronavirus Nonstructural Protein 9[J]. Virologica Sinica, 2005, 20(5): 552-554.

Citation: LIU Jia-sen, CHEN Shu-hong, LIU Huai-ran, PANG Hai, KONG Xian-gang, . Prokaryotic Expression of SARS Coronavirus Nonstructural Protein 9 .VIROLOGICA SINICA, 2005, 20(5) : 552-554.

SARS冠状病毒非结构蛋白Nsp的原核表达

刘家森 ^1,3 ,
陈淑红 ^1,3 ,
刘怀然 ¹ ,
孟庆文 ¹ ,
庞海 ^2* ,
孔宪刚 ^1*

1.
1.黑龙江哈尔滨中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室 2.清华大学医学院北京 3.东北农业大学动物医学学院

摘要
- SARS冠状病毒
- , 复制酶
- , 表达

Prokaryotic Expression of SARS Coronavirus Nonstructural Protein 9

LIU Jia-sen ^1,3 ,
CHEN Shu-hong ^1,3 ,
LIU Huai-ran ¹ ,
PANG Hai ¹ ,
KONG Xian-gang ^2* ,
^1*

Abstract

The gene of nonstructural protein 9 of SARS-coronavirus(SARS-CoV) was amplified using PCR from the product derivated from reverse transcription of SARS-CoV genome RNA,and was inserted into the multiple cloning sites of the expression vector pGEX-6p-1.Recombinant strain induced with IPTG expressed the specific soluble protein.The nsp9 protein was harvested and purified by affinity chromatography and processed by prescission protease.Polyclonal serum against the nsp9 protein was raised in rabbit.
- SARS coronavirus（SARS-CoV）
- , Replicase
- , Expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Prokaryotic Expression of SARS Coronavirus Nonstructural Protein 9

Abstract: The gene of nonstructural protein 9 of SARS-coronavirus(SARS-CoV) was amplified using PCR from the product derivated from reverse transcription of SARS-CoV genome RNA,and was inserted into the multiple cloning sites of the expression vector pGEX-6p-1.Recombinant strain induced with IPTG expressed the specific soluble protein.The nsp9 protein was harvested and purified by affinity chromatography and processed by prescission protease.Polyclonal serum against the nsp9 protein was raised in rabbit.