Study on a Transgenic Cell Line Stable Expression Gly145Arg Mutation HBsAg

TIAN Yong-jun; QIN Li; ZHANG Zheng-mao; LI Lei; LEI Yan-chang; YU Zhi-qun; ZHAO Xi-ping; YANG Dong-liang*

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December 2005

TIAN Yong-jun, QIN Li, ZHANG Zheng-mao, LI Lei, LEI Yan-chang, YU Zhi-qun, ZHAO Xi-ping and YANG Dong-liang*. Study on a Transgenic Cell Line Stable Expression Gly145Arg Mutation HBsAg[J]. Virologica Sinica, 2005, 20(6): 574-577.

Citation: TIAN Yong-jun, QIN Li, ZHANG Zheng-mao, LI Lei, LEI Yan-chang, YU Zhi-qun, ZHAO Xi-ping, YANG Dong-liang*. Study on a Transgenic Cell Line Stable Expression Gly145Arg Mutation HBsAg .VIROLOGICA SINICA, 2005, 20(6) : 574-577.

稳定表达乙型肝炎病毒G145R变异表面抗原细胞系的建立

1.
华中科技大学同济医学院附属同济医院临床免疫研究室感染学教研室湖北武汉

摘要

构建可以在真核细胞表达G145R HBsAg的重组质粒PCI-HBs145。用此质粒转染Hela细胞,经克隆化培养以及G418筛选,建立稳定表达G145R HBsAg的细胞系。ELISA检测表明表达G145R HBsAg与野生型HBsAg 在免疫反应性上有较大的差异。生物学性状研究结果表明稳定表达G145R HBsAg的2A8细胞纯度好,遗传性状稳定。
- 乙型肝炎病毒
- , 表面抗原
- , 变异
- , 转基因细胞系

Study on a Transgenic Cell Line Stable Expression Gly145Arg Mutation HBsAg

Abstract

A recombinant plasmid named pCI-HBs145 containing a fragment of G145R mutate S gene of Hepatitis B virus was constructed. Seven HeLa cell lines stablely expressing the protein of G145R HBsAg were developed by G418 selection after transfecting with the PCI-HBs145. Biological characteristics of one of the 7 cell lines, namely, 2A8 were studied. The immunoreactivity of G145R HBsAg expressed by 2A8 was different from wild type HBsAg. The genetic stability and purity of the cell population were confirmed. The kinetics and optimal conditions for mutation HBsAg secretion from 2A8 cells were studied.
- Hepatitis B
- , surface antigen（HBsAg）
- , Variant
- , Cell line

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on a Transgenic Cell Line Stable Expression Gly145Arg Mutation HBsAg

Abstract: A recombinant plasmid named pCI-HBs145 containing a fragment of G145R mutate S gene of Hepatitis B virus was constructed. Seven HeLa cell lines stablely expressing the protein of G145R HBsAg were developed by G418 selection after transfecting with the PCI-HBs145. Biological characteristics of one of the 7 cell lines, namely, 2A8 were studied. The immunoreactivity of G145R HBsAg expressed by 2A8 was different from wild type HBsAg. The genetic stability and purity of the cell population were confirmed. The kinetics and optimal conditions for mutation HBsAg secretion from 2A8 cells were studied.