Construction and Immunogenicity of Recombinant Adenovirus Expressing the M protein of Porcine Re- productive and Respiratory Syndrome Virus in Mice

TANG Jing-yuan; JIANG Ping*; JIANG Wen-ming; MA Su; LI Yu-feng

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December 2005

TANG Jing-yuan, JIANG Ping*, JIANG Wen-ming, MA Su and LI Yu-feng. Construction and Immunogenicity of Recombinant Adenovirus Expressing the M protein of Porcine Re- productive and Respiratory Syndrome Virus in Mice[J]. Virologica Sinica, 2005, 20(6): 618-622.

Citation: TANG Jing-yuan, JIANG Ping*, JIANG Wen-ming, MA Su, LI Yu-feng. Construction and Immunogenicity of Recombinant Adenovirus Expressing the M protein of Porcine Re- productive and Respiratory Syndrome Virus in Mice .VIROLOGICA SINICA, 2005, 20(6) : 618-622.

表达PRRSV M蛋白重组腺病毒的构建及其免疫特性研究

1.
南京农业大学农业部动物疫病诊断与免疫重点开放实验室江苏南京

摘要

本研究通过RT-PCR方法扩增猪繁殖与呼吸综合征病毒(PRRSV)S1株的M蛋白基因,将其克隆重组到人血清5型腺病毒载体中,转染293细胞,制备重组腺病毒rAd-M。RT-PCR和IFA方法鉴定,结果表明rAd-M可表达M基因的mRNA和M蛋白。纯化的rAd-M重组腺病毒经293细胞连续传25代,滴度稳定为107.8 TCID50／ mL。动物免疫试验结果表明,该重组腺病毒rAd-M能够刺激机体产生PRRSV的特异性抗体免疫和细胞免疫应答反应,从而为PRRSV结构蛋白功能及其基因工程疫苗研究奠定了基础。
- 重组腺病毒
- , 免疫反应
- , PRRSV
- , M蛋白

Construction and Immunogenicity of Recombinant Adenovirus Expressing the M protein of Porcine Re- productive and Respiratory Syndrome Virus in Mice

Abstract

To screen out vaccine candidate against PRRSV, adenovirus vector was used to express the M protein of Porcine reproductive and respiratory syndrome virus (PRRSV). A recombinant adenovirus was constructed and the expression of the M protein was identified by RT-PCR and indirect immunofluorescence assay (IFA). The purified recombinant adenovirus M (rAd-M) was passaged 25 times in 293A cells. The titer of stocks of rAd-M was stably 107.8\CID50/mL. Furthermore, the recombinant M protein adenovirus could induce PRRSV specific humoral immunity and cell mediated immunity in mice. It indicated that the recombinant adenovirus expressing the main structural proteins of PRRSV is a potentially viable candidate vaccine against PRRSV.
- Recombinant adenovirus
- , Immunity
- , PRRSV
- , M protein.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction and Immunogenicity of Recombinant Adenovirus Expressing the M protein of Porcine Re- productive and Respiratory Syndrome Virus in Mice

Abstract: To screen out vaccine candidate against PRRSV, adenovirus vector was used to express the M protein of Porcine reproductive and respiratory syndrome virus (PRRSV). A recombinant adenovirus was constructed and the expression of the M protein was identified by RT-PCR and indirect immunofluorescence assay (IFA). The purified recombinant adenovirus M (rAd-M) was passaged 25 times in 293A cells. The titer of stocks of rAd-M was stably 107.8\CID50/mL. Furthermore, the recombinant M protein adenovirus could induce PRRSV specific humoral immunity and cell mediated immunity in mice. It indicated that the recombinant adenovirus expressing the main structural proteins of PRRSV is a potentially viable candidate vaccine against PRRSV.