The Transcription Profiles of Nine HaSNPV Genes

DAI Wen-tao; DENG Fei; WANG Hua-lin; YUAN Li; HU Zhi-hong

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February 2006

DAI Wen-tao, DENG Fei, WANG Hua-lin, YUAN Li and HU Zhi-hong. The Transcription Profiles of Nine HaSNPV Genes[J]. Virologica Sinica, 2006, 21(1): 47-51.

Citation: DAI Wen-tao, DENG Fei, WANG Hua-lin, YUAN Li, HU Zhi-hong. The Transcription Profiles of Nine HaSNPV Genes .VIROLOGICA SINICA, 2006, 21(1) : 47-51.

HaSNPV的9个基因的转录谱分析

代文涛 ^1,2 ,
邓菲 ¹ ,
王华林 ¹ ,
袁丽 ¹ ,
胡志红 ^1*,,

1.
1. 中国科学院武汉病毒研究所，病毒学国家重点实验室，中国科学院分子病毒学重点实验室，无脊椎动物病毒学联合开放实验室，湖北武汉 430071
2.
中国科学院研究生院，北京 100039

通讯作者： 胡志红,

摘要

使用反转录和实时荧光定量PCR技术，我们对HaSNPV的几个预期的早期基因、早晚期基因、晚期基因、极晚期基因的转录时相进行分析，结果表明：这些基因的起始转录时间与其自身的启动子类型基本是一致的。但是预期的早期基因pkip晚期才开始转录；预期的早晚期基因ha107早期不转录，仅在晚期转录。这些基因的转录水平一般都在病毒感染细胞72 h后达到最高，并且极晚期基因polyhedrin的转录水平明显高于其它基因。Iap2的转录水平仅次于polyhedrin，表明它可能是一个功能基因。与AcMNPV的p10不同，在HaSNPV/HzAM1系统中p10的转录水平并不高。
- HaSNPV
- , 实时荧光定量PCR
- , 转录谱

The Transcription Profiles of Nine HaSNPV Genes

DAI Wen-tao ^1,2 ,
DENG Fei ¹ ,
WANG Hua-lin ¹ ,
YUAN Li ¹ ,
HU Zhi-hong ^1*,,

1.
1. State Key Laboratory of Virology, Key Laboratory of Molecular Virology, and Joint-Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2.
Graduate School of Chinese Academy of Sciences, Beijing 100039, China

Corresponding author: HU Zhi-hong,

Abstract

The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes the transcriptional start point was coincident with the prediction of their promoter. But the predicted early phase gene pkip started to be transcribed at late phase, and the predicted early and late phase gene ha107 was only transcribed at late phase. At 72hpi, the transcription level of these genes was generally the highest. The transcription level of polyhedrin was obviously higher than the other genes. The iap2 was the second highly transcribed gene, indicating that it might be functional. The HaSNPV p10, unlike its homologue in AcMNPV, was not highly transcribed.
- HaSNPV
- , Real-time quantitative PCR
- , Transcription profile

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Transcription Profiles of Nine HaSNPV Genes

Corresponding author: HU Zhi-hong,

1. 1. State Key Laboratory of Virology, Key Laboratory of Molecular Virology, and Joint-Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China

Abstract: The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes the transcriptional start point was coincident with the prediction of their promoter. But the predicted early phase gene pkip started to be transcribed at late phase, and the predicted early and late phase gene ha107 was only transcribed at late phase. At 72hpi, the transcription level of these genes was generally the highest. The transcription level of polyhedrin was obviously higher than the other genes. The iap2 was the second highly transcribed gene, indicating that it might be functional. The HaSNPV p10, unlike its homologue in AcMNPV, was not highly transcribed.